Cigarette smoke decreases MARCO expression in macrophages: Implication in Mycoplasma pneumoniae infection

Misbah Baqir, Chiung-Zuei Chen, Richard J. Martin, Jyoti Thaikoottathil, Stephanie R. Case, Maisha N. Minor, Russell Bowler, Hong Wei Chu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bacterial infections including Mycoplasma pneumoniae (Mp) are a major cause of exacerbations in chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is the leading cause of COPD, and affects the function of alveolar macrophages that act as the first line of defense against the invading respiratory pathogens. Macrophages express a transmembrane receptor called macrophage receptor with collagenous structure (MARCO) that is involved in the clearance of microorganisms. Whether CS down-regulates MARCO and eventually decreases the clearance of Mp has not been investigated. We utilized human monocytic cell line (THP-1)-derived macrophages to examine the effects of CS extract (CSE) on MARCO expression and Mp growth. Specifically, macrophages were pre-exposed to CSE for 6 h, and then infected with or without Mp for 2 h. MARCO was examined at both mRNA and protein levels by using real-time PCR and immunofluorescent staining, respectively. Mp in the supernatants was quantified by quantitative culture. In addition, a neutralizing MARCO antibody was added to macrophages to test if blockade of MARCO impaired Mp clearance. We found that CSE significantly decreased MARCO expression in a dose-dependant manner at 6 h post-CSE. Mp levels in CSE-treated cells were higher than those in non-CSE-treated cells, indicating a decreased pathogen clearance. Additionally, neutralizing MARCO in macrophages markedly increased Mp levels. Our results indicate that cigarette smoke exposure down-regulates MARCO expression in macrophages, which may be in part responsible for impaired bacterial (e.g., Mp) clearance.

Original languageEnglish
Pages (from-to)1604-1610
Number of pages7
JournalRespiratory Medicine
Volume102
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

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Mycoplasma pneumoniae
Smoke
Tobacco Products
Macrophages
Infection
Cell Extracts
Chronic Obstructive Pulmonary Disease
Down-Regulation
Alveolar Macrophages
Bacterial Infections

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Baqir, M., Chen, C-Z., Martin, R. J., Thaikoottathil, J., Case, S. R., Minor, M. N., ... Chu, H. W. (2008). Cigarette smoke decreases MARCO expression in macrophages: Implication in Mycoplasma pneumoniae infection. Respiratory Medicine, 102(11), 1604-1610. https://doi.org/10.1016/j.rmed.2008.05.002
Baqir, Misbah ; Chen, Chiung-Zuei ; Martin, Richard J. ; Thaikoottathil, Jyoti ; Case, Stephanie R. ; Minor, Maisha N. ; Bowler, Russell ; Chu, Hong Wei. / Cigarette smoke decreases MARCO expression in macrophages : Implication in Mycoplasma pneumoniae infection. In: Respiratory Medicine. 2008 ; Vol. 102, No. 11. pp. 1604-1610.
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abstract = "Bacterial infections including Mycoplasma pneumoniae (Mp) are a major cause of exacerbations in chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is the leading cause of COPD, and affects the function of alveolar macrophages that act as the first line of defense against the invading respiratory pathogens. Macrophages express a transmembrane receptor called macrophage receptor with collagenous structure (MARCO) that is involved in the clearance of microorganisms. Whether CS down-regulates MARCO and eventually decreases the clearance of Mp has not been investigated. We utilized human monocytic cell line (THP-1)-derived macrophages to examine the effects of CS extract (CSE) on MARCO expression and Mp growth. Specifically, macrophages were pre-exposed to CSE for 6 h, and then infected with or without Mp for 2 h. MARCO was examined at both mRNA and protein levels by using real-time PCR and immunofluorescent staining, respectively. Mp in the supernatants was quantified by quantitative culture. In addition, a neutralizing MARCO antibody was added to macrophages to test if blockade of MARCO impaired Mp clearance. We found that CSE significantly decreased MARCO expression in a dose-dependant manner at 6 h post-CSE. Mp levels in CSE-treated cells were higher than those in non-CSE-treated cells, indicating a decreased pathogen clearance. Additionally, neutralizing MARCO in macrophages markedly increased Mp levels. Our results indicate that cigarette smoke exposure down-regulates MARCO expression in macrophages, which may be in part responsible for impaired bacterial (e.g., Mp) clearance.",
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Baqir, M, Chen, C-Z, Martin, RJ, Thaikoottathil, J, Case, SR, Minor, MN, Bowler, R & Chu, HW 2008, 'Cigarette smoke decreases MARCO expression in macrophages: Implication in Mycoplasma pneumoniae infection', Respiratory Medicine, vol. 102, no. 11, pp. 1604-1610. https://doi.org/10.1016/j.rmed.2008.05.002

Cigarette smoke decreases MARCO expression in macrophages : Implication in Mycoplasma pneumoniae infection. / Baqir, Misbah; Chen, Chiung-Zuei; Martin, Richard J.; Thaikoottathil, Jyoti; Case, Stephanie R.; Minor, Maisha N.; Bowler, Russell; Chu, Hong Wei.

In: Respiratory Medicine, Vol. 102, No. 11, 01.11.2008, p. 1604-1610.

Research output: Contribution to journalArticle

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T1 - Cigarette smoke decreases MARCO expression in macrophages

T2 - Implication in Mycoplasma pneumoniae infection

AU - Baqir, Misbah

AU - Chen, Chiung-Zuei

AU - Martin, Richard J.

AU - Thaikoottathil, Jyoti

AU - Case, Stephanie R.

AU - Minor, Maisha N.

AU - Bowler, Russell

AU - Chu, Hong Wei

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N2 - Bacterial infections including Mycoplasma pneumoniae (Mp) are a major cause of exacerbations in chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is the leading cause of COPD, and affects the function of alveolar macrophages that act as the first line of defense against the invading respiratory pathogens. Macrophages express a transmembrane receptor called macrophage receptor with collagenous structure (MARCO) that is involved in the clearance of microorganisms. Whether CS down-regulates MARCO and eventually decreases the clearance of Mp has not been investigated. We utilized human monocytic cell line (THP-1)-derived macrophages to examine the effects of CS extract (CSE) on MARCO expression and Mp growth. Specifically, macrophages were pre-exposed to CSE for 6 h, and then infected with or without Mp for 2 h. MARCO was examined at both mRNA and protein levels by using real-time PCR and immunofluorescent staining, respectively. Mp in the supernatants was quantified by quantitative culture. In addition, a neutralizing MARCO antibody was added to macrophages to test if blockade of MARCO impaired Mp clearance. We found that CSE significantly decreased MARCO expression in a dose-dependant manner at 6 h post-CSE. Mp levels in CSE-treated cells were higher than those in non-CSE-treated cells, indicating a decreased pathogen clearance. Additionally, neutralizing MARCO in macrophages markedly increased Mp levels. Our results indicate that cigarette smoke exposure down-regulates MARCO expression in macrophages, which may be in part responsible for impaired bacterial (e.g., Mp) clearance.

AB - Bacterial infections including Mycoplasma pneumoniae (Mp) are a major cause of exacerbations in chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is the leading cause of COPD, and affects the function of alveolar macrophages that act as the first line of defense against the invading respiratory pathogens. Macrophages express a transmembrane receptor called macrophage receptor with collagenous structure (MARCO) that is involved in the clearance of microorganisms. Whether CS down-regulates MARCO and eventually decreases the clearance of Mp has not been investigated. We utilized human monocytic cell line (THP-1)-derived macrophages to examine the effects of CS extract (CSE) on MARCO expression and Mp growth. Specifically, macrophages were pre-exposed to CSE for 6 h, and then infected with or without Mp for 2 h. MARCO was examined at both mRNA and protein levels by using real-time PCR and immunofluorescent staining, respectively. Mp in the supernatants was quantified by quantitative culture. In addition, a neutralizing MARCO antibody was added to macrophages to test if blockade of MARCO impaired Mp clearance. We found that CSE significantly decreased MARCO expression in a dose-dependant manner at 6 h post-CSE. Mp levels in CSE-treated cells were higher than those in non-CSE-treated cells, indicating a decreased pathogen clearance. Additionally, neutralizing MARCO in macrophages markedly increased Mp levels. Our results indicate that cigarette smoke exposure down-regulates MARCO expression in macrophages, which may be in part responsible for impaired bacterial (e.g., Mp) clearance.

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