Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells

Jin Tang Chen, Torng Sen Lin, Kuan Chih Chow, Hsuan Hua Huang, Shiow Her Chiou, Shu Fen Chiang, Hong Chen Chen, Tzu Lin Chuang, Tze Yi Lin, Chih Yi Chen

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HCF expression in ATII cells and lung cancer cells.

Original languageEnglish
Pages (from-to)264-273
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume34
Issue number3
DOIs
Publication statusPublished - 2006 Mar

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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