Cinnamophilin reduces oxidative damage and protects against transient focal cerebral ischemia in mice

E. Jian Lee, Hung Yi Chen, Ming Yang Lee, Tsung Ying Chen, Yun Shang Hsu, Yu Ling Hu, Guan Liang Chang, Tian Shung Wu

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Acute neuroprotective effects of cinnamophilin (CINN; (8R, 8′S)-4, 4′-dihydroxy-3, 3′-dimethoxy-7-oxo-8, 8′-neolignan), a novel antioxidant and free radical scavenger, were studied in a mouse model of transient middle cerebral artery (MCA) occlusion. CINN was administered intraperitoneally either 15 min before (pretreatment) or 2 h after the onset of MCA occlusion (postischemic treatment). Relative to vehicle-treated controls, animals pretreated with CINN, at 20-80 mg/kg, had significant reductions in brain infarction by 33-46% and improvements in neurobehavioral outcome. Postischemic administration with CINN (80 mg/kg) also significantly reduced brain infarction by 43% and ameliorated neurobehavioral deficits. Additionally, CINN administration significantly attenuated in situ accumulation of superoxide anions (O2-) in the boundary zones of infarct at 4 h after reperfusion. Consequently, CINN-treated animals exhibited significantly decreased levels of oxidative damage, as assessed by immunopositive reactions for 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 4-hydroxynonenal (4-HNE), and the resultant inflammatory reactions at 24 h postinsult. It is concluded that CINN effectively reduced brain infarction and improved neurobehavioral outcome following a short-term recovery period after severe transient focal cerebral ischemia in mice. The finding of a decreased extent of reactive oxygen species and oxidative damage observed with CINN treatment highlights that its antioxidant and radical scavenging ability is contributory.

Original languageEnglish
Pages (from-to)495-510
Number of pages16
JournalFree Radical Biology and Medicine
Volume39
Issue number4
DOIs
Publication statusPublished - 2005 Aug 15

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

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