Circulating adhesion molecules and neutral endopeptidase enzymuria in patients with urolithiasis and hydronephrosis

Ho-Shiang Huang, Jun Chen, Chau Fong Chen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objectives. To measure the concentrations of adhesion molecules (intercellular adhesion molecule-1 [ICAM-1], E-selectin, and L-selectin) in the serum of patients with renal stones and patients with hydronephrosis caused by obstructive ureteral stones. Renal tubular enzymes were examined from their urine samples to evaluate whether neutral endopeptidase (NEP) behaved as traditional tubular enzyme markers (N-acetyl-β-glucosaminidase [NAG] and β-galactosidase [β-GAL]) in their disease state. Methods. Three groups were studied. Group 1 included 15 normal volunteers, group 2 included 12 patients with ureteral stones and ipsilateral hydronephrosis, and group 3 included 17 patients with renal stones in one kidney without hydronephrosis or hydrocalycosis. A single, overnight fasting blood and urine sample was collected from each subject. Serum levels of ICAM-1, E-selectin, and L-selectin were measured by enzyme-linked immunosorbent assay. NEP, NAG, and β-GAL were measured from urine samples, and the enzyme activities were expressed per gram of creatinine. Results. Serum levels of ICAM-1 were higher in groups 2 and 3 (522 ± 95 and 329 ± 42 ng/mL, respectively), but the differences were only significant between group 2 and group 1 (263 ± 32 ng/mL) and group 2 and group 3 (P <0.05). Serum levels of L-selectin were lower in group 3 and were significantly different when compared with groups 1 and 2 (P <0.05). The serum levels of E-selectin were not significantly different among these three groups. Urinary levels of NEP were lower in group 2, although the levels of NAG and β-GAL were more elevated than in group 1. Conclusions. Serum levels of ICAM-1 were elevated in patients with unilateral hydronephrosis caused by a ureteral stone, and E-selectin and L-selectin levels did not change significantly. These findings suggest that ICAM-1 may play a role in renal immune injury to ureteral obstruction. The urinary NEP values were lower in patients with hydronephrosis caused by ureteral stones, and the traditional lysosomal enzymes were increased. The lower urinary NEP values might suggest impairment of ipsilateral renal function. Renal stones per se resulted in no significant changes in serum adhesion molecule levels, although the levels of L-selectin were significantly decreased. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)961-965
Number of pages5
JournalUrology
Volume55
Issue number6
DOIs
Publication statusPublished - 2000 Jun 1

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Neprilysin
Urolithiasis
Hydronephrosis
L-Selectin
Intercellular Adhesion Molecule-1
E-Selectin
Kidney
Hexosaminidases
Serum
Urine
Enzymes
Galactosidases
Ureteral Obstruction
Fasting
Creatinine
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

@article{d7564b12e6674abb84822b6495fcf3ad,
title = "Circulating adhesion molecules and neutral endopeptidase enzymuria in patients with urolithiasis and hydronephrosis",
abstract = "Objectives. To measure the concentrations of adhesion molecules (intercellular adhesion molecule-1 [ICAM-1], E-selectin, and L-selectin) in the serum of patients with renal stones and patients with hydronephrosis caused by obstructive ureteral stones. Renal tubular enzymes were examined from their urine samples to evaluate whether neutral endopeptidase (NEP) behaved as traditional tubular enzyme markers (N-acetyl-β-glucosaminidase [NAG] and β-galactosidase [β-GAL]) in their disease state. Methods. Three groups were studied. Group 1 included 15 normal volunteers, group 2 included 12 patients with ureteral stones and ipsilateral hydronephrosis, and group 3 included 17 patients with renal stones in one kidney without hydronephrosis or hydrocalycosis. A single, overnight fasting blood and urine sample was collected from each subject. Serum levels of ICAM-1, E-selectin, and L-selectin were measured by enzyme-linked immunosorbent assay. NEP, NAG, and β-GAL were measured from urine samples, and the enzyme activities were expressed per gram of creatinine. Results. Serum levels of ICAM-1 were higher in groups 2 and 3 (522 ± 95 and 329 ± 42 ng/mL, respectively), but the differences were only significant between group 2 and group 1 (263 ± 32 ng/mL) and group 2 and group 3 (P <0.05). Serum levels of L-selectin were lower in group 3 and were significantly different when compared with groups 1 and 2 (P <0.05). The serum levels of E-selectin were not significantly different among these three groups. Urinary levels of NEP were lower in group 2, although the levels of NAG and β-GAL were more elevated than in group 1. Conclusions. Serum levels of ICAM-1 were elevated in patients with unilateral hydronephrosis caused by a ureteral stone, and E-selectin and L-selectin levels did not change significantly. These findings suggest that ICAM-1 may play a role in renal immune injury to ureteral obstruction. The urinary NEP values were lower in patients with hydronephrosis caused by ureteral stones, and the traditional lysosomal enzymes were increased. The lower urinary NEP values might suggest impairment of ipsilateral renal function. Renal stones per se resulted in no significant changes in serum adhesion molecule levels, although the levels of L-selectin were significantly decreased. Copyright (C) 2000 Elsevier Science Inc.",
author = "Ho-Shiang Huang and Jun Chen and Chen, {Chau Fong}",
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Circulating adhesion molecules and neutral endopeptidase enzymuria in patients with urolithiasis and hydronephrosis. / Huang, Ho-Shiang; Chen, Jun; Chen, Chau Fong.

In: Urology, Vol. 55, No. 6, 01.06.2000, p. 961-965.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Circulating adhesion molecules and neutral endopeptidase enzymuria in patients with urolithiasis and hydronephrosis

AU - Huang, Ho-Shiang

AU - Chen, Jun

AU - Chen, Chau Fong

PY - 2000/6/1

Y1 - 2000/6/1

N2 - Objectives. To measure the concentrations of adhesion molecules (intercellular adhesion molecule-1 [ICAM-1], E-selectin, and L-selectin) in the serum of patients with renal stones and patients with hydronephrosis caused by obstructive ureteral stones. Renal tubular enzymes were examined from their urine samples to evaluate whether neutral endopeptidase (NEP) behaved as traditional tubular enzyme markers (N-acetyl-β-glucosaminidase [NAG] and β-galactosidase [β-GAL]) in their disease state. Methods. Three groups were studied. Group 1 included 15 normal volunteers, group 2 included 12 patients with ureteral stones and ipsilateral hydronephrosis, and group 3 included 17 patients with renal stones in one kidney without hydronephrosis or hydrocalycosis. A single, overnight fasting blood and urine sample was collected from each subject. Serum levels of ICAM-1, E-selectin, and L-selectin were measured by enzyme-linked immunosorbent assay. NEP, NAG, and β-GAL were measured from urine samples, and the enzyme activities were expressed per gram of creatinine. Results. Serum levels of ICAM-1 were higher in groups 2 and 3 (522 ± 95 and 329 ± 42 ng/mL, respectively), but the differences were only significant between group 2 and group 1 (263 ± 32 ng/mL) and group 2 and group 3 (P <0.05). Serum levels of L-selectin were lower in group 3 and were significantly different when compared with groups 1 and 2 (P <0.05). The serum levels of E-selectin were not significantly different among these three groups. Urinary levels of NEP were lower in group 2, although the levels of NAG and β-GAL were more elevated than in group 1. Conclusions. Serum levels of ICAM-1 were elevated in patients with unilateral hydronephrosis caused by a ureteral stone, and E-selectin and L-selectin levels did not change significantly. These findings suggest that ICAM-1 may play a role in renal immune injury to ureteral obstruction. The urinary NEP values were lower in patients with hydronephrosis caused by ureteral stones, and the traditional lysosomal enzymes were increased. The lower urinary NEP values might suggest impairment of ipsilateral renal function. Renal stones per se resulted in no significant changes in serum adhesion molecule levels, although the levels of L-selectin were significantly decreased. Copyright (C) 2000 Elsevier Science Inc.

AB - Objectives. To measure the concentrations of adhesion molecules (intercellular adhesion molecule-1 [ICAM-1], E-selectin, and L-selectin) in the serum of patients with renal stones and patients with hydronephrosis caused by obstructive ureteral stones. Renal tubular enzymes were examined from their urine samples to evaluate whether neutral endopeptidase (NEP) behaved as traditional tubular enzyme markers (N-acetyl-β-glucosaminidase [NAG] and β-galactosidase [β-GAL]) in their disease state. Methods. Three groups were studied. Group 1 included 15 normal volunteers, group 2 included 12 patients with ureteral stones and ipsilateral hydronephrosis, and group 3 included 17 patients with renal stones in one kidney without hydronephrosis or hydrocalycosis. A single, overnight fasting blood and urine sample was collected from each subject. Serum levels of ICAM-1, E-selectin, and L-selectin were measured by enzyme-linked immunosorbent assay. NEP, NAG, and β-GAL were measured from urine samples, and the enzyme activities were expressed per gram of creatinine. Results. Serum levels of ICAM-1 were higher in groups 2 and 3 (522 ± 95 and 329 ± 42 ng/mL, respectively), but the differences were only significant between group 2 and group 1 (263 ± 32 ng/mL) and group 2 and group 3 (P <0.05). Serum levels of L-selectin were lower in group 3 and were significantly different when compared with groups 1 and 2 (P <0.05). The serum levels of E-selectin were not significantly different among these three groups. Urinary levels of NEP were lower in group 2, although the levels of NAG and β-GAL were more elevated than in group 1. Conclusions. Serum levels of ICAM-1 were elevated in patients with unilateral hydronephrosis caused by a ureteral stone, and E-selectin and L-selectin levels did not change significantly. These findings suggest that ICAM-1 may play a role in renal immune injury to ureteral obstruction. The urinary NEP values were lower in patients with hydronephrosis caused by ureteral stones, and the traditional lysosomal enzymes were increased. The lower urinary NEP values might suggest impairment of ipsilateral renal function. Renal stones per se resulted in no significant changes in serum adhesion molecule levels, although the levels of L-selectin were significantly decreased. Copyright (C) 2000 Elsevier Science Inc.

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