Clinical and molecular features of MDR livestock-associated MRSA ST9 with staphylococcal cassette chromosome mecXII in humans

Chih Jung Chen, Tsai Ling Yang Lauderdale, Chin Te Lu, Yu Yu Chuang, Chien Chang Yang, Ting Shu Wu, Chun Yi Lee, Min Chi Lu, Wen Chien Ko, Yhu Chering Huang

Research output: Contribution to journalArticle

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Abstract

Objectives: Clonal complex (CC) 9 is a prevalent livestock-associated (LA) MRSA clone in Asia whose pathogenicity in humans remains unknown. Methods: In 2012, we identified a patient with CC9-MRSA infection linked to livestock. After screening 3328 clinical MRSA isolates from a national database, eight isolates (0.24%) collected between 1998 and 2012 were further confirmed to be of CC9. The detailed molecular features of the nine human CC9 strains and phylogenetic relatedness to animal CC9 strains were characterized with WGS. The antibiotic susceptibilities were determined and the clinical information was abstracted frommedical records. Results: WGS grouped the CC9 strains into two clades, which were respectively associated with distinct toxome profiles, resistance gene profiles and staphylococcal cassette chromosomes (SCCmecXII for 7 isolates and SCCmecVT for 2 isolates). The SCCmecXII strains were phylogenetically related to animal CC9-MRSA strains, negative for Panton-Valentine leucocidin and 100% resistant to ciprofloxacin, erythromycin, clindamycin, gentamicin and tigecycline. Four of the seven SCCmecXII isolates were associated with invasive diseases including bacteraemia leading to death (2) and osteomyelitis (2). Two SCCmecXII isolates were from patients with exposure to pigs before development of the MRSA diseases. Conclusions: The CC9-SCCmecXII MRSA prevailing in pigs in Asia ismultidrug resistant and potentially pathogenic to humans. It is critical to continuously monitor the local epidemiology of MRSA and implement effective control measures to limit the spread of LA-MRSA between animals, to humans and in healthcare facilities.

Original languageEnglish
Article numberdkx357
Pages (from-to)33-40
Number of pages8
JournalJournal of Antimicrobial Chemotherapy
Volume73
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

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Livestock
Methicillin-Resistant Staphylococcus aureus
Chromosomes
Swine
Chromosomes, Human, Pair 7
Clindamycin
Osteomyelitis
Erythromycin
Ciprofloxacin
Bacteremia
Gentamicins
Virulence
Epidemiology
Clone Cells
Databases
Anti-Bacterial Agents
Delivery of Health Care
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Chen, C. J., Lauderdale, T. L. Y., Lu, C. T., Chuang, Y. Y., Yang, C. C., Wu, T. S., ... Huang, Y. C. (2018). Clinical and molecular features of MDR livestock-associated MRSA ST9 with staphylococcal cassette chromosome mecXII in humans. Journal of Antimicrobial Chemotherapy, 73(1), 33-40. [dkx357]. https://doi.org/10.1093/jac/dkx357
Chen, Chih Jung ; Lauderdale, Tsai Ling Yang ; Lu, Chin Te ; Chuang, Yu Yu ; Yang, Chien Chang ; Wu, Ting Shu ; Lee, Chun Yi ; Lu, Min Chi ; Ko, Wen Chien ; Huang, Yhu Chering. / Clinical and molecular features of MDR livestock-associated MRSA ST9 with staphylococcal cassette chromosome mecXII in humans. In: Journal of Antimicrobial Chemotherapy. 2018 ; Vol. 73, No. 1. pp. 33-40.
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abstract = "Objectives: Clonal complex (CC) 9 is a prevalent livestock-associated (LA) MRSA clone in Asia whose pathogenicity in humans remains unknown. Methods: In 2012, we identified a patient with CC9-MRSA infection linked to livestock. After screening 3328 clinical MRSA isolates from a national database, eight isolates (0.24{\%}) collected between 1998 and 2012 were further confirmed to be of CC9. The detailed molecular features of the nine human CC9 strains and phylogenetic relatedness to animal CC9 strains were characterized with WGS. The antibiotic susceptibilities were determined and the clinical information was abstracted frommedical records. Results: WGS grouped the CC9 strains into two clades, which were respectively associated with distinct toxome profiles, resistance gene profiles and staphylococcal cassette chromosomes (SCCmecXII for 7 isolates and SCCmecVT for 2 isolates). The SCCmecXII strains were phylogenetically related to animal CC9-MRSA strains, negative for Panton-Valentine leucocidin and 100{\%} resistant to ciprofloxacin, erythromycin, clindamycin, gentamicin and tigecycline. Four of the seven SCCmecXII isolates were associated with invasive diseases including bacteraemia leading to death (2) and osteomyelitis (2). Two SCCmecXII isolates were from patients with exposure to pigs before development of the MRSA diseases. Conclusions: The CC9-SCCmecXII MRSA prevailing in pigs in Asia ismultidrug resistant and potentially pathogenic to humans. It is critical to continuously monitor the local epidemiology of MRSA and implement effective control measures to limit the spread of LA-MRSA between animals, to humans and in healthcare facilities.",
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Chen, CJ, Lauderdale, TLY, Lu, CT, Chuang, YY, Yang, CC, Wu, TS, Lee, CY, Lu, MC, Ko, WC & Huang, YC 2018, 'Clinical and molecular features of MDR livestock-associated MRSA ST9 with staphylococcal cassette chromosome mecXII in humans', Journal of Antimicrobial Chemotherapy, vol. 73, no. 1, dkx357, pp. 33-40. https://doi.org/10.1093/jac/dkx357

Clinical and molecular features of MDR livestock-associated MRSA ST9 with staphylococcal cassette chromosome mecXII in humans. / Chen, Chih Jung; Lauderdale, Tsai Ling Yang; Lu, Chin Te; Chuang, Yu Yu; Yang, Chien Chang; Wu, Ting Shu; Lee, Chun Yi; Lu, Min Chi; Ko, Wen Chien; Huang, Yhu Chering.

In: Journal of Antimicrobial Chemotherapy, Vol. 73, No. 1, dkx357, 01.01.2018, p. 33-40.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Clinical and molecular features of MDR livestock-associated MRSA ST9 with staphylococcal cassette chromosome mecXII in humans

AU - Chen, Chih Jung

AU - Lauderdale, Tsai Ling Yang

AU - Lu, Chin Te

AU - Chuang, Yu Yu

AU - Yang, Chien Chang

AU - Wu, Ting Shu

AU - Lee, Chun Yi

AU - Lu, Min Chi

AU - Ko, Wen Chien

AU - Huang, Yhu Chering

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Y1 - 2018/1/1

N2 - Objectives: Clonal complex (CC) 9 is a prevalent livestock-associated (LA) MRSA clone in Asia whose pathogenicity in humans remains unknown. Methods: In 2012, we identified a patient with CC9-MRSA infection linked to livestock. After screening 3328 clinical MRSA isolates from a national database, eight isolates (0.24%) collected between 1998 and 2012 were further confirmed to be of CC9. The detailed molecular features of the nine human CC9 strains and phylogenetic relatedness to animal CC9 strains were characterized with WGS. The antibiotic susceptibilities were determined and the clinical information was abstracted frommedical records. Results: WGS grouped the CC9 strains into two clades, which were respectively associated with distinct toxome profiles, resistance gene profiles and staphylococcal cassette chromosomes (SCCmecXII for 7 isolates and SCCmecVT for 2 isolates). The SCCmecXII strains were phylogenetically related to animal CC9-MRSA strains, negative for Panton-Valentine leucocidin and 100% resistant to ciprofloxacin, erythromycin, clindamycin, gentamicin and tigecycline. Four of the seven SCCmecXII isolates were associated with invasive diseases including bacteraemia leading to death (2) and osteomyelitis (2). Two SCCmecXII isolates were from patients with exposure to pigs before development of the MRSA diseases. Conclusions: The CC9-SCCmecXII MRSA prevailing in pigs in Asia ismultidrug resistant and potentially pathogenic to humans. It is critical to continuously monitor the local epidemiology of MRSA and implement effective control measures to limit the spread of LA-MRSA between animals, to humans and in healthcare facilities.

AB - Objectives: Clonal complex (CC) 9 is a prevalent livestock-associated (LA) MRSA clone in Asia whose pathogenicity in humans remains unknown. Methods: In 2012, we identified a patient with CC9-MRSA infection linked to livestock. After screening 3328 clinical MRSA isolates from a national database, eight isolates (0.24%) collected between 1998 and 2012 were further confirmed to be of CC9. The detailed molecular features of the nine human CC9 strains and phylogenetic relatedness to animal CC9 strains were characterized with WGS. The antibiotic susceptibilities were determined and the clinical information was abstracted frommedical records. Results: WGS grouped the CC9 strains into two clades, which were respectively associated with distinct toxome profiles, resistance gene profiles and staphylococcal cassette chromosomes (SCCmecXII for 7 isolates and SCCmecVT for 2 isolates). The SCCmecXII strains were phylogenetically related to animal CC9-MRSA strains, negative for Panton-Valentine leucocidin and 100% resistant to ciprofloxacin, erythromycin, clindamycin, gentamicin and tigecycline. Four of the seven SCCmecXII isolates were associated with invasive diseases including bacteraemia leading to death (2) and osteomyelitis (2). Two SCCmecXII isolates were from patients with exposure to pigs before development of the MRSA diseases. Conclusions: The CC9-SCCmecXII MRSA prevailing in pigs in Asia ismultidrug resistant and potentially pathogenic to humans. It is critical to continuously monitor the local epidemiology of MRSA and implement effective control measures to limit the spread of LA-MRSA between animals, to humans and in healthcare facilities.

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