Clinical significance of allelotype profiling for urothelial carcinoma

Tzong Shin Tzai, Helen H.W Chen, Shih-Huang Chan, Chung-Liang Ho, Yuh-Shyan Tsai, Hong-Lin Cheng, Yuan Chang Dai, Johnny Shinn Nan Lin, Wen-Horng Yang, Nan-Haw Chow

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objectives. To perform a global loss of heterozygosity (LOH) analysis on a cohort of urothelial carcinoma to investigate the clinical implication of specific chromosomal loss. Allelic deletions detected as LOH have been used to study the markers for carcinogenesis. Methods. We examined the allelic loss on 14 chromosomal regions in a total of 71 cases of urothelial carcinoma. The results were analyzed in relation to biologic indicators of urothelial carcinoma and the clinical outcome with a mean follow-up of 101 months. Results. The incidence of LOH in order of frequency was 9p (54.9%), 9q (49.3%), 13q (40.8%), 14q (40.8%), 10q (39.4%), 17p (39.4%), 8p (38.0%), 21q (36.6%), 11p (31.0%), 18q (23.9%), 4q (21.1%), 3p (16.9%), 6q (14.1%), and 1q (8.5%). Positive association with one of the indicators was observed in 3p, 9p, 9q, 10q, 14q, and 18q. The chromosomes that correlated with two biologic indicators were 4q, 6q, 11p, 17p, and 21q. Univariate analysis found that patients having combined 9p and 14q deleted tumors had particularly poor long-term survival compared with those with other patterns of chromosomal alterations (P = 0.01). In the multivariate model, nonpapillary tumors had a greater risk of recurrence, and stage classification was the only important indicator in predicting patient survival (P = 0.04). Conclusions. LOH assessment does not provide independent prognostic value compared with stage classification. However, chromosomes 4q, 6q, 9p, 11p, 14q, 17p, and 21q may harbor important tumor suppressor genes involved in the progression of urothelial carcinogenesis.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalUrology
Volume62
Issue number2
DOIs
Publication statusPublished - 2003 Aug 1

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Loss of Heterozygosity
Carcinoma
Carcinogenesis
Chromosomes
Survival
Tumor Suppressor Genes
Neoplasms
Recurrence
Incidence

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

@article{2237fe06e2a84518bc60d088db523c70,
title = "Clinical significance of allelotype profiling for urothelial carcinoma",
abstract = "Objectives. To perform a global loss of heterozygosity (LOH) analysis on a cohort of urothelial carcinoma to investigate the clinical implication of specific chromosomal loss. Allelic deletions detected as LOH have been used to study the markers for carcinogenesis. Methods. We examined the allelic loss on 14 chromosomal regions in a total of 71 cases of urothelial carcinoma. The results were analyzed in relation to biologic indicators of urothelial carcinoma and the clinical outcome with a mean follow-up of 101 months. Results. The incidence of LOH in order of frequency was 9p (54.9{\%}), 9q (49.3{\%}), 13q (40.8{\%}), 14q (40.8{\%}), 10q (39.4{\%}), 17p (39.4{\%}), 8p (38.0{\%}), 21q (36.6{\%}), 11p (31.0{\%}), 18q (23.9{\%}), 4q (21.1{\%}), 3p (16.9{\%}), 6q (14.1{\%}), and 1q (8.5{\%}). Positive association with one of the indicators was observed in 3p, 9p, 9q, 10q, 14q, and 18q. The chromosomes that correlated with two biologic indicators were 4q, 6q, 11p, 17p, and 21q. Univariate analysis found that patients having combined 9p and 14q deleted tumors had particularly poor long-term survival compared with those with other patterns of chromosomal alterations (P = 0.01). In the multivariate model, nonpapillary tumors had a greater risk of recurrence, and stage classification was the only important indicator in predicting patient survival (P = 0.04). Conclusions. LOH assessment does not provide independent prognostic value compared with stage classification. However, chromosomes 4q, 6q, 9p, 11p, 14q, 17p, and 21q may harbor important tumor suppressor genes involved in the progression of urothelial carcinogenesis.",
author = "Tzai, {Tzong Shin} and Chen, {Helen H.W} and Shih-Huang Chan and Chung-Liang Ho and Yuh-Shyan Tsai and Hong-Lin Cheng and Dai, {Yuan Chang} and Lin, {Johnny Shinn Nan} and Wen-Horng Yang and Nan-Haw Chow",
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Clinical significance of allelotype profiling for urothelial carcinoma. / Tzai, Tzong Shin; Chen, Helen H.W; Chan, Shih-Huang; Ho, Chung-Liang; Tsai, Yuh-Shyan; Cheng, Hong-Lin; Dai, Yuan Chang; Lin, Johnny Shinn Nan; Yang, Wen-Horng; Chow, Nan-Haw.

In: Urology, Vol. 62, No. 2, 01.08.2003, p. 378-384.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Clinical significance of allelotype profiling for urothelial carcinoma

AU - Tzai, Tzong Shin

AU - Chen, Helen H.W

AU - Chan, Shih-Huang

AU - Ho, Chung-Liang

AU - Tsai, Yuh-Shyan

AU - Cheng, Hong-Lin

AU - Dai, Yuan Chang

AU - Lin, Johnny Shinn Nan

AU - Yang, Wen-Horng

AU - Chow, Nan-Haw

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Objectives. To perform a global loss of heterozygosity (LOH) analysis on a cohort of urothelial carcinoma to investigate the clinical implication of specific chromosomal loss. Allelic deletions detected as LOH have been used to study the markers for carcinogenesis. Methods. We examined the allelic loss on 14 chromosomal regions in a total of 71 cases of urothelial carcinoma. The results were analyzed in relation to biologic indicators of urothelial carcinoma and the clinical outcome with a mean follow-up of 101 months. Results. The incidence of LOH in order of frequency was 9p (54.9%), 9q (49.3%), 13q (40.8%), 14q (40.8%), 10q (39.4%), 17p (39.4%), 8p (38.0%), 21q (36.6%), 11p (31.0%), 18q (23.9%), 4q (21.1%), 3p (16.9%), 6q (14.1%), and 1q (8.5%). Positive association with one of the indicators was observed in 3p, 9p, 9q, 10q, 14q, and 18q. The chromosomes that correlated with two biologic indicators were 4q, 6q, 11p, 17p, and 21q. Univariate analysis found that patients having combined 9p and 14q deleted tumors had particularly poor long-term survival compared with those with other patterns of chromosomal alterations (P = 0.01). In the multivariate model, nonpapillary tumors had a greater risk of recurrence, and stage classification was the only important indicator in predicting patient survival (P = 0.04). Conclusions. LOH assessment does not provide independent prognostic value compared with stage classification. However, chromosomes 4q, 6q, 9p, 11p, 14q, 17p, and 21q may harbor important tumor suppressor genes involved in the progression of urothelial carcinogenesis.

AB - Objectives. To perform a global loss of heterozygosity (LOH) analysis on a cohort of urothelial carcinoma to investigate the clinical implication of specific chromosomal loss. Allelic deletions detected as LOH have been used to study the markers for carcinogenesis. Methods. We examined the allelic loss on 14 chromosomal regions in a total of 71 cases of urothelial carcinoma. The results were analyzed in relation to biologic indicators of urothelial carcinoma and the clinical outcome with a mean follow-up of 101 months. Results. The incidence of LOH in order of frequency was 9p (54.9%), 9q (49.3%), 13q (40.8%), 14q (40.8%), 10q (39.4%), 17p (39.4%), 8p (38.0%), 21q (36.6%), 11p (31.0%), 18q (23.9%), 4q (21.1%), 3p (16.9%), 6q (14.1%), and 1q (8.5%). Positive association with one of the indicators was observed in 3p, 9p, 9q, 10q, 14q, and 18q. The chromosomes that correlated with two biologic indicators were 4q, 6q, 11p, 17p, and 21q. Univariate analysis found that patients having combined 9p and 14q deleted tumors had particularly poor long-term survival compared with those with other patterns of chromosomal alterations (P = 0.01). In the multivariate model, nonpapillary tumors had a greater risk of recurrence, and stage classification was the only important indicator in predicting patient survival (P = 0.04). Conclusions. LOH assessment does not provide independent prognostic value compared with stage classification. However, chromosomes 4q, 6q, 9p, 11p, 14q, 17p, and 21q may harbor important tumor suppressor genes involved in the progression of urothelial carcinogenesis.

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U2 - 10.1016/S0090-4295(03)00344-3

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