Clitocybe nuda activates dendritic cells and acts as A DNA vaccine adjuvant

Mei Hsing Chen, Wei Sung Li, Yun Sheng Lue, Ching Liang Chu, I. Hong Pan, Ching Huai Ko, Der Yuan Chen, Ching Hsiung Lin, Sheng Hao Lin, Chih Peng Chang, Chi Chen Lin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This work represents the first evaluation of the effects of water extract of C. nuda (WE-CN), an edible mushroom, on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. Our experimental results show that WE-CN could induce phenotypic maturation of DCs, as shown by the increased expression of MHC and costimulatory molecules. In addition, it also induced the proinflammatory cytokines expression on DCs and enhanced both the proliferation and IFN-γ secretion of allogenic T cells. Therefore, since WE-CN did not induce maturation of DCs generated from mice with mutated TLR-4 or TLR-2, suggesting that TLR4 and TLR2 might function as membrane receptors for WE-CN. Moreover, the mechanism of action of WE-CN may be mediated by increased phosphorylation of ERK, p38, and JNK mitogen-activated protein kinase (MAPK) and increased NF-B p65 activity, which are important signaling molecules downstream of TLR-4 and TLR-2. Finally, coimmunization of mice with WE-CN and a HER-2/neu DNA vaccine induced a HER-2/neu-specific Th1 response that resulted in significant inhibition of HER-2/neu overexpressing mouse bladder tumor (MBT-2) growth. These data suggest that WE-CN induces DC maturation through TLR-4 and/or TLR-2 and that WE-CN can be used as an adjuvant in cancer vaccine immunotherapy.

Original languageEnglish
Article number761454
JournalEvidence-based Complementary and Alternative Medicine
Volume2013
DOIs
Publication statusPublished - 2013 Sep 23

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DNA Vaccines
Dendritic Cells
Water
Cancer Vaccines
JNK Mitogen-Activated Protein Kinases
Agaricales
p38 Mitogen-Activated Protein Kinases
Urinary Bladder Neoplasms
Immunotherapy
Bone Marrow
Phosphorylation
Cytokines
T-Lymphocytes
Membranes
Growth

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

Chen, M. H., Li, W. S., Lue, Y. S., Chu, C. L., Pan, I. H., Ko, C. H., ... Lin, C. C. (2013). Clitocybe nuda activates dendritic cells and acts as A DNA vaccine adjuvant. Evidence-based Complementary and Alternative Medicine, 2013, [761454]. https://doi.org/10.1155/2013/761454
Chen, Mei Hsing ; Li, Wei Sung ; Lue, Yun Sheng ; Chu, Ching Liang ; Pan, I. Hong ; Ko, Ching Huai ; Chen, Der Yuan ; Lin, Ching Hsiung ; Lin, Sheng Hao ; Chang, Chih Peng ; Lin, Chi Chen. / Clitocybe nuda activates dendritic cells and acts as A DNA vaccine adjuvant. In: Evidence-based Complementary and Alternative Medicine. 2013 ; Vol. 2013.
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abstract = "This work represents the first evaluation of the effects of water extract of C. nuda (WE-CN), an edible mushroom, on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. Our experimental results show that WE-CN could induce phenotypic maturation of DCs, as shown by the increased expression of MHC and costimulatory molecules. In addition, it also induced the proinflammatory cytokines expression on DCs and enhanced both the proliferation and IFN-γ secretion of allogenic T cells. Therefore, since WE-CN did not induce maturation of DCs generated from mice with mutated TLR-4 or TLR-2, suggesting that TLR4 and TLR2 might function as membrane receptors for WE-CN. Moreover, the mechanism of action of WE-CN may be mediated by increased phosphorylation of ERK, p38, and JNK mitogen-activated protein kinase (MAPK) and increased NF-B p65 activity, which are important signaling molecules downstream of TLR-4 and TLR-2. Finally, coimmunization of mice with WE-CN and a HER-2/neu DNA vaccine induced a HER-2/neu-specific Th1 response that resulted in significant inhibition of HER-2/neu overexpressing mouse bladder tumor (MBT-2) growth. These data suggest that WE-CN induces DC maturation through TLR-4 and/or TLR-2 and that WE-CN can be used as an adjuvant in cancer vaccine immunotherapy.",
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Chen, MH, Li, WS, Lue, YS, Chu, CL, Pan, IH, Ko, CH, Chen, DY, Lin, CH, Lin, SH, Chang, CP & Lin, CC 2013, 'Clitocybe nuda activates dendritic cells and acts as A DNA vaccine adjuvant', Evidence-based Complementary and Alternative Medicine, vol. 2013, 761454. https://doi.org/10.1155/2013/761454

Clitocybe nuda activates dendritic cells and acts as A DNA vaccine adjuvant. / Chen, Mei Hsing; Li, Wei Sung; Lue, Yun Sheng; Chu, Ching Liang; Pan, I. Hong; Ko, Ching Huai; Chen, Der Yuan; Lin, Ching Hsiung; Lin, Sheng Hao; Chang, Chih Peng; Lin, Chi Chen.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2013, 761454, 23.09.2013.

Research output: Contribution to journalArticle

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T1 - Clitocybe nuda activates dendritic cells and acts as A DNA vaccine adjuvant

AU - Chen, Mei Hsing

AU - Li, Wei Sung

AU - Lue, Yun Sheng

AU - Chu, Ching Liang

AU - Pan, I. Hong

AU - Ko, Ching Huai

AU - Chen, Der Yuan

AU - Lin, Ching Hsiung

AU - Lin, Sheng Hao

AU - Chang, Chih Peng

AU - Lin, Chi Chen

PY - 2013/9/23

Y1 - 2013/9/23

N2 - This work represents the first evaluation of the effects of water extract of C. nuda (WE-CN), an edible mushroom, on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. Our experimental results show that WE-CN could induce phenotypic maturation of DCs, as shown by the increased expression of MHC and costimulatory molecules. In addition, it also induced the proinflammatory cytokines expression on DCs and enhanced both the proliferation and IFN-γ secretion of allogenic T cells. Therefore, since WE-CN did not induce maturation of DCs generated from mice with mutated TLR-4 or TLR-2, suggesting that TLR4 and TLR2 might function as membrane receptors for WE-CN. Moreover, the mechanism of action of WE-CN may be mediated by increased phosphorylation of ERK, p38, and JNK mitogen-activated protein kinase (MAPK) and increased NF-B p65 activity, which are important signaling molecules downstream of TLR-4 and TLR-2. Finally, coimmunization of mice with WE-CN and a HER-2/neu DNA vaccine induced a HER-2/neu-specific Th1 response that resulted in significant inhibition of HER-2/neu overexpressing mouse bladder tumor (MBT-2) growth. These data suggest that WE-CN induces DC maturation through TLR-4 and/or TLR-2 and that WE-CN can be used as an adjuvant in cancer vaccine immunotherapy.

AB - This work represents the first evaluation of the effects of water extract of C. nuda (WE-CN), an edible mushroom, on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. Our experimental results show that WE-CN could induce phenotypic maturation of DCs, as shown by the increased expression of MHC and costimulatory molecules. In addition, it also induced the proinflammatory cytokines expression on DCs and enhanced both the proliferation and IFN-γ secretion of allogenic T cells. Therefore, since WE-CN did not induce maturation of DCs generated from mice with mutated TLR-4 or TLR-2, suggesting that TLR4 and TLR2 might function as membrane receptors for WE-CN. Moreover, the mechanism of action of WE-CN may be mediated by increased phosphorylation of ERK, p38, and JNK mitogen-activated protein kinase (MAPK) and increased NF-B p65 activity, which are important signaling molecules downstream of TLR-4 and TLR-2. Finally, coimmunization of mice with WE-CN and a HER-2/neu DNA vaccine induced a HER-2/neu-specific Th1 response that resulted in significant inhibition of HER-2/neu overexpressing mouse bladder tumor (MBT-2) growth. These data suggest that WE-CN induces DC maturation through TLR-4 and/or TLR-2 and that WE-CN can be used as an adjuvant in cancer vaccine immunotherapy.

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