Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster

Jiaqian Zhu, Ching-Hao Teng, Chao Fu Chang, Ching Dong Chang, Kenneth W. Simpson, Christina Wei, Patrick McDonough, Sean McDonough, Yung Fu Chang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The urease gene cluster from Helicobacter bizzozeronii was cloned and sequenced. A genomic library was constructed in a λ -ZAPII vector using TSP 5091-digested H. bizzozeronii chromosomal DNA. Four overlapping recombinant bacteriophages carrying the H. bizzozeronii urease genes were identified by using a fragment of H. bizzozeronii ureB as a probe. Sequence analysis of two clones (pHB1 and pHB3) revealed seven open reading frames encoding proteins with predicted masses of 26.5, 60.3, 21.7, 19.5, 28.6, 21.7 and 29.6 kDa representing the structural genes, Urease A and B and its accessory genes, urease I, E, F, G and H, respectively. In addition, three open reading frames upstream of the ureA gene encoding a putative tRNA transferase, a putative Glucose inhibited division protein B (GidB) and a protein with unknown function were also identified. A clone (pHB5) containing a complete urease gene cluster was constructed. The homologue analysis revealed that UreA polypeptide exhibited 64-90% identity to that of Helicobacter heilmanii, Helicobacter felis, Helicobacter pylori, Helicobacter mustelae and Helicobacter hepaticus. UreB polypeptides exhibited 76.8-96% identity to that of H. heilmanii, H. felis, H. pylori, H. mustelae and H. hepaticus. The UreI, E, F, G and H also showed 44-86% identity to that of H. pylori. Among these accessory genes, UreE had a lowest percentage identity to that of H. pylori.

Original languageEnglish
Pages (from-to)321-331
Number of pages11
JournalMitochondrial DNA
Volume13
Issue number6
DOIs
Publication statusPublished - 2002 Dec 1

Fingerprint

Helicobacter
Urease
Multigene Family
Organism Cloning
Helicobacter pylori
Helicobacter mustelae
Helicobacter felis
Helicobacter hepaticus
Genes
Open Reading Frames
Urea
Clone Cells
Peptides
Genomic Library
Transferases
Transfer RNA
Bacteriophages
Sequence Analysis
Proteins
Glucose

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Zhu, J., Teng, C-H., Chang, C. F., Chang, C. D., Simpson, K. W., Wei, C., ... Chang, Y. F. (2002). Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster. Mitochondrial DNA, 13(6), 321-331. https://doi.org/10.1080/1042517021000039230
Zhu, Jiaqian ; Teng, Ching-Hao ; Chang, Chao Fu ; Chang, Ching Dong ; Simpson, Kenneth W. ; Wei, Christina ; McDonough, Patrick ; McDonough, Sean ; Chang, Yung Fu. / Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster. In: Mitochondrial DNA. 2002 ; Vol. 13, No. 6. pp. 321-331.
@article{0c33d817dccc4361866ef1fcc393232f,
title = "Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster",
abstract = "The urease gene cluster from Helicobacter bizzozeronii was cloned and sequenced. A genomic library was constructed in a λ -ZAPII vector using TSP 5091-digested H. bizzozeronii chromosomal DNA. Four overlapping recombinant bacteriophages carrying the H. bizzozeronii urease genes were identified by using a fragment of H. bizzozeronii ureB as a probe. Sequence analysis of two clones (pHB1 and pHB3) revealed seven open reading frames encoding proteins with predicted masses of 26.5, 60.3, 21.7, 19.5, 28.6, 21.7 and 29.6 kDa representing the structural genes, Urease A and B and its accessory genes, urease I, E, F, G and H, respectively. In addition, three open reading frames upstream of the ureA gene encoding a putative tRNA transferase, a putative Glucose inhibited division protein B (GidB) and a protein with unknown function were also identified. A clone (pHB5) containing a complete urease gene cluster was constructed. The homologue analysis revealed that UreA polypeptide exhibited 64-90{\%} identity to that of Helicobacter heilmanii, Helicobacter felis, Helicobacter pylori, Helicobacter mustelae and Helicobacter hepaticus. UreB polypeptides exhibited 76.8-96{\%} identity to that of H. heilmanii, H. felis, H. pylori, H. mustelae and H. hepaticus. The UreI, E, F, G and H also showed 44-86{\%} identity to that of H. pylori. Among these accessory genes, UreE had a lowest percentage identity to that of H. pylori.",
author = "Jiaqian Zhu and Ching-Hao Teng and Chang, {Chao Fu} and Chang, {Ching Dong} and Simpson, {Kenneth W.} and Christina Wei and Patrick McDonough and Sean McDonough and Chang, {Yung Fu}",
year = "2002",
month = "12",
day = "1",
doi = "10.1080/1042517021000039230",
language = "English",
volume = "13",
pages = "321--331",
journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",
issn = "1940-1736",
publisher = "Informa Healthcare",
number = "6",

}

Zhu, J, Teng, C-H, Chang, CF, Chang, CD, Simpson, KW, Wei, C, McDonough, P, McDonough, S & Chang, YF 2002, 'Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster', Mitochondrial DNA, vol. 13, no. 6, pp. 321-331. https://doi.org/10.1080/1042517021000039230

Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster. / Zhu, Jiaqian; Teng, Ching-Hao; Chang, Chao Fu; Chang, Ching Dong; Simpson, Kenneth W.; Wei, Christina; McDonough, Patrick; McDonough, Sean; Chang, Yung Fu.

In: Mitochondrial DNA, Vol. 13, No. 6, 01.12.2002, p. 321-331.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cloning and characterization of a Helicobacter bizzozeronii urease gene cluster

AU - Zhu, Jiaqian

AU - Teng, Ching-Hao

AU - Chang, Chao Fu

AU - Chang, Ching Dong

AU - Simpson, Kenneth W.

AU - Wei, Christina

AU - McDonough, Patrick

AU - McDonough, Sean

AU - Chang, Yung Fu

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The urease gene cluster from Helicobacter bizzozeronii was cloned and sequenced. A genomic library was constructed in a λ -ZAPII vector using TSP 5091-digested H. bizzozeronii chromosomal DNA. Four overlapping recombinant bacteriophages carrying the H. bizzozeronii urease genes were identified by using a fragment of H. bizzozeronii ureB as a probe. Sequence analysis of two clones (pHB1 and pHB3) revealed seven open reading frames encoding proteins with predicted masses of 26.5, 60.3, 21.7, 19.5, 28.6, 21.7 and 29.6 kDa representing the structural genes, Urease A and B and its accessory genes, urease I, E, F, G and H, respectively. In addition, three open reading frames upstream of the ureA gene encoding a putative tRNA transferase, a putative Glucose inhibited division protein B (GidB) and a protein with unknown function were also identified. A clone (pHB5) containing a complete urease gene cluster was constructed. The homologue analysis revealed that UreA polypeptide exhibited 64-90% identity to that of Helicobacter heilmanii, Helicobacter felis, Helicobacter pylori, Helicobacter mustelae and Helicobacter hepaticus. UreB polypeptides exhibited 76.8-96% identity to that of H. heilmanii, H. felis, H. pylori, H. mustelae and H. hepaticus. The UreI, E, F, G and H also showed 44-86% identity to that of H. pylori. Among these accessory genes, UreE had a lowest percentage identity to that of H. pylori.

AB - The urease gene cluster from Helicobacter bizzozeronii was cloned and sequenced. A genomic library was constructed in a λ -ZAPII vector using TSP 5091-digested H. bizzozeronii chromosomal DNA. Four overlapping recombinant bacteriophages carrying the H. bizzozeronii urease genes were identified by using a fragment of H. bizzozeronii ureB as a probe. Sequence analysis of two clones (pHB1 and pHB3) revealed seven open reading frames encoding proteins with predicted masses of 26.5, 60.3, 21.7, 19.5, 28.6, 21.7 and 29.6 kDa representing the structural genes, Urease A and B and its accessory genes, urease I, E, F, G and H, respectively. In addition, three open reading frames upstream of the ureA gene encoding a putative tRNA transferase, a putative Glucose inhibited division protein B (GidB) and a protein with unknown function were also identified. A clone (pHB5) containing a complete urease gene cluster was constructed. The homologue analysis revealed that UreA polypeptide exhibited 64-90% identity to that of Helicobacter heilmanii, Helicobacter felis, Helicobacter pylori, Helicobacter mustelae and Helicobacter hepaticus. UreB polypeptides exhibited 76.8-96% identity to that of H. heilmanii, H. felis, H. pylori, H. mustelae and H. hepaticus. The UreI, E, F, G and H also showed 44-86% identity to that of H. pylori. Among these accessory genes, UreE had a lowest percentage identity to that of H. pylori.

UR - http://www.scopus.com/inward/record.url?scp=0036920017&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036920017&partnerID=8YFLogxK

U2 - 10.1080/1042517021000039230

DO - 10.1080/1042517021000039230

M3 - Article

C2 - 12652903

AN - SCOPUS:0036920017

VL - 13

SP - 321

EP - 331

JO - DNA Sequence - Journal of DNA Sequencing and Mapping

JF - DNA Sequence - Journal of DNA Sequencing and Mapping

SN - 1940-1736

IS - 6

ER -