TY - JOUR
T1 - Cloning and characterization of a myosin from characean alga, the fastest motor protein in the world
AU - Kashiyama, Taku
AU - Kimura, Naohiro
AU - Mimura, Tetsuro
AU - Yamamoto, Keiichi
PY - 2000
Y1 - 2000
N2 - In characean algae, very rapid cytoplasmic streaming is generated by sliding movement of an unconventional myosin on fixed actin cables. The speed of this sliding movement is the fastest among many molecular motors known so far. We have cloned a set of overlapping cDNAs encoding the heavy chain of this myosin by immunoscreening with antibody raised against characean myosin. The molecular mass of this heavy chain is 248 kDa, and the protein has a conserved motor domain, six IQ motifs, an extensive α-helical coiled-coil domain, and a C-terminal globular domain. Phylogenetic analysis suggested that this myosin belongs to class XI.
AB - In characean algae, very rapid cytoplasmic streaming is generated by sliding movement of an unconventional myosin on fixed actin cables. The speed of this sliding movement is the fastest among many molecular motors known so far. We have cloned a set of overlapping cDNAs encoding the heavy chain of this myosin by immunoscreening with antibody raised against characean myosin. The molecular mass of this heavy chain is 248 kDa, and the protein has a conserved motor domain, six IQ motifs, an extensive α-helical coiled-coil domain, and a C-terminal globular domain. Phylogenetic analysis suggested that this myosin belongs to class XI.
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U2 - 10.1093/oxfordjournals.jbchem.a022699
DO - 10.1093/oxfordjournals.jbchem.a022699
M3 - Article
C2 - 10833276
AN - SCOPUS:0033935268
SN - 0021-924X
VL - 127
SP - 1065
EP - 1070
JO - Journal of biochemistry
JF - Journal of biochemistry
IS - 6
ER -