Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo

Ming Chyuan Chen, Hong Yi Gong, Ching Yi Cheng, Jia Pey Wang, Jiann-Ruey Hong, Jen Leih Wu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. To elucidate the embryonic roles of Bcl-2 family proteins, we cloned and characterized the first zebrafish Bcl-2 family protein, zfMcl-1a. Zebrafish Mcl-1a shows the highest homology to rat Mcl-1 and contains several conserved BH domains of the Bcl-2 family proteins. It also contains a nuclear localization signal (NLS). Using EGFP reporter analysis, we verified the nuclear localization of zfMcl-1a. Deletion of the NLS resulted in distribution of the fusion protein in the cytoplasm. Northern blot analysis indicated that zfMcl-1a mRNA is 1.5 kb and was expressed in oocytes and throughout embryonic development. Notably, the expression of zfMcl-1a transcript was significantly downregulated during gastrulation. These results suggest that zfMcl-1a is a novel nuclear Bcl-2 family protein and is likely to play an important role in zebrafish oogenesis and embryogenesis.

Original languageEnglish
Pages (from-to)725-731
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume279
Issue number2
DOIs
Publication statusPublished - 2000 Jan 1

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Cloning
Zebrafish
Organism Cloning
Embryonic Structures
Embryonic Development
Nuclear Localization Signals
Proteins
Oogenesis
Gastrulation
Northern Blotting
Oocytes
Vertebrates
Rats
Cytoplasm
Fusion reactions
Down-Regulation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chen, Ming Chyuan ; Gong, Hong Yi ; Cheng, Ching Yi ; Wang, Jia Pey ; Hong, Jiann-Ruey ; Wu, Jen Leih. / Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 279, No. 2. pp. 725-731.
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Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo. / Chen, Ming Chyuan; Gong, Hong Yi; Cheng, Ching Yi; Wang, Jia Pey; Hong, Jiann-Ruey; Wu, Jen Leih.

In: Biochemical and Biophysical Research Communications, Vol. 279, No. 2, 01.01.2000, p. 725-731.

Research output: Contribution to journalArticle

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