TY - JOUR
T1 - Cloning and characterization of a small-size peptide Zfra that regulates the cytotoxic function of tumor necrosis factor by interacting with JNK1
AU - Hsu, Li Jin
AU - Schultz, Lori
AU - Mattison, Jeffrey
AU - Lin, Yee Shin
AU - Chang, Nan Shan
N1 - Funding Information:
Research was supported in part by the American Heart Association, the Department of Defense (DAMD17-03-1-0736), and the Guthrie Foundation for Education and Research. L.J.H. was supported by the Ministry of Education, Taiwan, ROC (91-B-FA09-1-4). We thank Dr. V. Ruiz-Velasco for rat brains, and Drs. T. Miki and S. Aaronson for the λpCEV27 vector.
PY - 2005/2/11
Y1 - 2005/2/11
N2 - By cDNA library screening, here we isolated an unusual gene transcript encoding a 31-amino-acid zinc finger-like peptide that regulates apoptosis (named Zfra). Northern blotting and RT/PCR showed the transcript is abundant in spleen but absent in several prostate and breast cancer cells. When stably expressed in L929 fibroblasts, Zfra conferred resistance to the cytotoxic effects of TNF and FasL. In contrast, transiently expressed Zfra could enhance or inhibit the cytotoxicity of overexpressed death domain proteins TRADD, FADD, and RIP of the TNF signaling pathway. By GST pull-down assay and co-immunoprecipitation, TNF and UV light were shown to induce Zfra to rapidly self-associate and bind JNK1. While JNK1 is a downstream effector of the TNF signaling, Zfra regulation of the TNF cytotoxic function is likely due to its interaction, in part, with JNK1.
AB - By cDNA library screening, here we isolated an unusual gene transcript encoding a 31-amino-acid zinc finger-like peptide that regulates apoptosis (named Zfra). Northern blotting and RT/PCR showed the transcript is abundant in spleen but absent in several prostate and breast cancer cells. When stably expressed in L929 fibroblasts, Zfra conferred resistance to the cytotoxic effects of TNF and FasL. In contrast, transiently expressed Zfra could enhance or inhibit the cytotoxicity of overexpressed death domain proteins TRADD, FADD, and RIP of the TNF signaling pathway. By GST pull-down assay and co-immunoprecipitation, TNF and UV light were shown to induce Zfra to rapidly self-associate and bind JNK1. While JNK1 is a downstream effector of the TNF signaling, Zfra regulation of the TNF cytotoxic function is likely due to its interaction, in part, with JNK1.
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U2 - 10.1016/j.bbrc.2004.12.025
DO - 10.1016/j.bbrc.2004.12.025
M3 - Article
C2 - 15629131
AN - SCOPUS:11144307264
SN - 0006-291X
VL - 327
SP - 415
EP - 423
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -