Cloning and characterization of a transforming growth factor β1-induced anti-apoptotic adhesion protein TIF2

Gregory B. Carey, Nan-Shan Chang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Transforming growth factor-β (TGF-β) antagonizes the cytotoxic function of tumor necrosis factor (TNF). By differential display and library screening, we isolated a murine TIF2 (TGF-β-induced factor 2) cDNA, encoding a putative 15-kDa membrane adhesion protein, which possesses an RGD sequence at the extracellular region. When TNF-sensitive murine L929 fibroblasts were stably transfected with TIF2 cDNA, these cells significantly resisted TNF killing. In contrast, L929 cells, which stably expressed the TIF2 antisense mRNA, acquired enhanced TNF susceptibility. Calculated EC50 values, i.e., the amount of TNF needed for killing 50% cells, are 10, 55, and 1.5 ng/ml, respectively, for vector control, sense transfectant, and antisense transfectant. TGF-β1 rapidly induces TIF2 gene expression (~1 hr), which correlates with time-related acquisition of TNF-resistance in TGF-β1-treated L929 cells. Notably, TIF2 gene expression is markedly increased in human breast cancer and lymphoid leukemia cells, compared to normal human cells, suggesting its potential role in cancer development. Together, the anti-apoptotic function of TIF2 is responsible in part for TGF-β-mediated protection of L929 cells against TNF cytotoxicity.

Original languageEnglish
Pages (from-to)283-286
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume249
Issue number1
DOIs
Publication statusPublished - 1998 Aug 10

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Apoptosis Regulatory Proteins
Cloning
Transforming Growth Factors
Organism Cloning
Adhesion
Tumor Necrosis Factor-alpha
Proteins
Gene expression
Complementary DNA
Lymphoid Leukemia
Gene Expression
Cytoprotection
Fibroblasts
Cytotoxicity
Screening
Membrane Proteins
Display devices
Cells
Lymphocytes
Breast Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Transforming growth factor-β (TGF-β) antagonizes the cytotoxic function of tumor necrosis factor (TNF). By differential display and library screening, we isolated a murine TIF2 (TGF-β-induced factor 2) cDNA, encoding a putative 15-kDa membrane adhesion protein, which possesses an RGD sequence at the extracellular region. When TNF-sensitive murine L929 fibroblasts were stably transfected with TIF2 cDNA, these cells significantly resisted TNF killing. In contrast, L929 cells, which stably expressed the TIF2 antisense mRNA, acquired enhanced TNF susceptibility. Calculated EC50 values, i.e., the amount of TNF needed for killing 50{\%} cells, are 10, 55, and 1.5 ng/ml, respectively, for vector control, sense transfectant, and antisense transfectant. TGF-β1 rapidly induces TIF2 gene expression (~1 hr), which correlates with time-related acquisition of TNF-resistance in TGF-β1-treated L929 cells. Notably, TIF2 gene expression is markedly increased in human breast cancer and lymphoid leukemia cells, compared to normal human cells, suggesting its potential role in cancer development. Together, the anti-apoptotic function of TIF2 is responsible in part for TGF-β-mediated protection of L929 cells against TNF cytotoxicity.",
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Cloning and characterization of a transforming growth factor β1-induced anti-apoptotic adhesion protein TIF2. / Carey, Gregory B.; Chang, Nan-Shan.

In: Biochemical and Biophysical Research Communications, Vol. 249, No. 1, 10.08.1998, p. 283-286.

Research output: Contribution to journalArticle

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