Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene

M. S. Chang; J. McNinch; R. Basu; J. Shutter; R. Y. Hsu; C. Perkins; V. Mar; S. Suggs; A. Welcher; L. Li; H. Lu; T. Bartley; P. Hunt; F. Martin; B. Samal; J. Bogenberger

doi:10.1074/jbc.270.2.511

Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene

M. S. Chang, J. McNinch, R. Basu, J. Shutter, R. Y. Hsu, C. Perkins, V. Mar, S. Suggs, A. Welcher, L. Li, H. Lu, T. Bartley, P. Hunt, F. Martin, B. Samal, J. Bogenberger

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

The megakaryocyte growth and development factor (MGDF) is a cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. In this study, we describe the genomic structure of the human MGDF gene. The MGDF gene was found to consist of seven exons and six introns spanning 8 kilobases. The protein is encoded by exons 3 through 7. The human MGDF gene has been mapped to chromosome 3q26.3. In addition to the previously described full-length cDNA, two cDNA variants were isolated from human fetal liver. Comparison of these two cDNA sequences with the genomic sequence indicates that they arise by differential splicing.

Original language	English
Pages (from-to)	511-514
Number of pages	4
Journal	Journal of Biological Chemistry
Volume	270
Issue number	2
DOIs	https://doi.org/10.1074/jbc.270.2.511
Publication status	Published - 1995

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1074/jbc.270.2.511

Cite this

Chang, M. S., McNinch, J., Basu, R., Shutter, J., Hsu, R. Y., Perkins, C., Mar, V., Suggs, S., Welcher, A., Li, L., Lu, H., Bartley, T., Hunt, P., Martin, F., Samal, B., & Bogenberger, J. (1995). Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene. Journal of Biological Chemistry, 270(2), 511-514. https://doi.org/10.1074/jbc.270.2.511

@article{438222d901f04021aa9496a2fc97b645,

title = "Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene",

abstract = "The megakaryocyte growth and development factor (MGDF) is a cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. In this study, we describe the genomic structure of the human MGDF gene. The MGDF gene was found to consist of seven exons and six introns spanning 8 kilobases. The protein is encoded by exons 3 through 7. The human MGDF gene has been mapped to chromosome 3q26.3. In addition to the previously described full-length cDNA, two cDNA variants were isolated from human fetal liver. Comparison of these two cDNA sequences with the genomic sequence indicates that they arise by differential splicing.",

author = "Chang, {M. S.} and J. McNinch and R. Basu and J. Shutter and Hsu, {R. Y.} and C. Perkins and V. Mar and S. Suggs and A. Welcher and L. Li and H. Lu and T. Bartley and P. Hunt and F. Martin and B. Samal and J. Bogenberger",

year = "1995",

doi = "10.1074/jbc.270.2.511",

language = "English",

volume = "270",

pages = "511--514",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "2",

}

Chang, MS, McNinch, J, Basu, R, Shutter, J, Hsu, RY, Perkins, C, Mar, V, Suggs, S, Welcher, A, Li, L, Lu, H, Bartley, T, Hunt, P, Martin, F, Samal, B & Bogenberger, J 1995, 'Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene', Journal of Biological Chemistry, vol. 270, no. 2, pp. 511-514. https://doi.org/10.1074/jbc.270.2.511

TY - JOUR

T1 - Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene

AU - Chang, M. S.

AU - McNinch, J.

AU - Basu, R.

AU - Shutter, J.

AU - Hsu, R. Y.

AU - Perkins, C.

AU - Mar, V.

AU - Suggs, S.

AU - Welcher, A.

AU - Li, L.

AU - Lu, H.

AU - Bartley, T.

AU - Hunt, P.

AU - Martin, F.

AU - Samal, B.

AU - Bogenberger, J.

PY - 1995

Y1 - 1995

N2 - The megakaryocyte growth and development factor (MGDF) is a cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. In this study, we describe the genomic structure of the human MGDF gene. The MGDF gene was found to consist of seven exons and six introns spanning 8 kilobases. The protein is encoded by exons 3 through 7. The human MGDF gene has been mapped to chromosome 3q26.3. In addition to the previously described full-length cDNA, two cDNA variants were isolated from human fetal liver. Comparison of these two cDNA sequences with the genomic sequence indicates that they arise by differential splicing.

AB - The megakaryocyte growth and development factor (MGDF) is a cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. In this study, we describe the genomic structure of the human MGDF gene. The MGDF gene was found to consist of seven exons and six introns spanning 8 kilobases. The protein is encoded by exons 3 through 7. The human MGDF gene has been mapped to chromosome 3q26.3. In addition to the previously described full-length cDNA, two cDNA variants were isolated from human fetal liver. Comparison of these two cDNA sequences with the genomic sequence indicates that they arise by differential splicing.

UR - http://www.scopus.com/inward/record.url?scp=0028898571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028898571&partnerID=8YFLogxK

U2 - 10.1074/jbc.270.2.511

DO - 10.1074/jbc.270.2.511

M3 - Article

C2 - 7822271

AN - SCOPUS:0028898571

SN - 0021-9258

VL - 270

SP - 511

EP - 514

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 2

ER -

Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this