TY - JOUR
T1 - Cloning and characterization of zfBLP1, a Bcl-XL homologue from the zebrafish, Danio rerio
AU - Chen, Ming Chyuan
AU - Gong, Hong Yi
AU - Cheng, Ching Yi
AU - Wang, Jia Pey
AU - Hong, Jiann Ruey
AU - Wu, Jen Leih
N1 - Funding Information:
The authors are grateful to Dr. A.-F. Knowles (San Diego State University, USA) for her comments on the manuscript, Ms. C.-C. Lin for the DNA sequencing work at Academia Sinica, and to Ms. M.-H. Wu for her secretarial assistance. We also thank all colleagues in the Laboratory of Marine Biology and Marine Biotechnology, Institute of Zoology, Academia Sinica, for their assistance and constructive discussion. This work was supported by a grant from the National Science Council (NSC89-2311-B-001-055), Taiwan to J.-L.W.
PY - 2001/5/28
Y1 - 2001/5/28
N2 - The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. We report here the cloning and characterization of a novel zebrafish Bcl-2 family protein, zfBLP1. The zfBLP1 cDNA is 1942 nucleotides long, encoding a polypeptide of 238 amino acids. The primary sequence of zfBLP1 shares 50% identity to human Bcl-XL, and contains all four conserved BH domains of the Bcl-2 family proteins. Primary sequence analysis identified a consensus ER retention signal at the C-terminal end of zfBLP1. Northern blot analysis indicated that there were two major and two minor zfBLP1 mRNA species expressed during embryonic development. Among the two major mRNA species, the short one, approx. 3 kb in size, was expressed throughout embryonic development, while the long one, approx. 7 kb long, was not detectable until the gastrula stage. These results suggest that zfBLP1 is a novel Bcl-2 family protein under complicated regulations, and is likely to play an important role in zebrafish oogenesis and embryogenesis.
AB - The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. We report here the cloning and characterization of a novel zebrafish Bcl-2 family protein, zfBLP1. The zfBLP1 cDNA is 1942 nucleotides long, encoding a polypeptide of 238 amino acids. The primary sequence of zfBLP1 shares 50% identity to human Bcl-XL, and contains all four conserved BH domains of the Bcl-2 family proteins. Primary sequence analysis identified a consensus ER retention signal at the C-terminal end of zfBLP1. Northern blot analysis indicated that there were two major and two minor zfBLP1 mRNA species expressed during embryonic development. Among the two major mRNA species, the short one, approx. 3 kb in size, was expressed throughout embryonic development, while the long one, approx. 7 kb long, was not detectable until the gastrula stage. These results suggest that zfBLP1 is a novel Bcl-2 family protein under complicated regulations, and is likely to play an important role in zebrafish oogenesis and embryogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0035962923&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035962923&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(01)00209-3
DO - 10.1016/S0167-4781(01)00209-3
M3 - Article
C2 - 11406282
AN - SCOPUS:0035962923
VL - 1519
SP - 127
EP - 133
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1-2
ER -