Cloning and characterization of zfBLP1, a Bcl-XL homologue from the zebrafish, Danio rerio

Ming Chyuan Chen, Hong Yi Gong, Ching Yi Cheng, Jia Pey Wang, Jiann Ruey Hong, Jen Leih Wu

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. We report here the cloning and characterization of a novel zebrafish Bcl-2 family protein, zfBLP1. The zfBLP1 cDNA is 1942 nucleotides long, encoding a polypeptide of 238 amino acids. The primary sequence of zfBLP1 shares 50% identity to human Bcl-XL, and contains all four conserved BH domains of the Bcl-2 family proteins. Primary sequence analysis identified a consensus ER retention signal at the C-terminal end of zfBLP1. Northern blot analysis indicated that there were two major and two minor zfBLP1 mRNA species expressed during embryonic development. Among the two major mRNA species, the short one, approx. 3 kb in size, was expressed throughout embryonic development, while the long one, approx. 7 kb long, was not detectable until the gastrula stage. These results suggest that zfBLP1 is a novel Bcl-2 family protein under complicated regulations, and is likely to play an important role in zebrafish oogenesis and embryogenesis.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1519
Issue number1-2
DOIs
Publication statusPublished - 2001 May 28

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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