Cloning and genetic variability of a HindIII DNA in Acrossocheilus paradoxus repetitive (Cyprinidae)

Wen Luan Wu, Jiang Ping Wang, Mei Chen Tseng, Tzen Yuh Chiang

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10 Citations (Scopus)

Abstract

Thirty clones of a highly repetitive HindIII fragment of DNA from seven populations of Acrossocheilus paradoxus (Cyprinidae) were isolated and sequenced. The fragment represents a tandemly repeated sequence, with a monomeric unit of 270 bp, amounting to 0.08-0.10% of the fish genome. Higher units of this monomer appear as a ladder in Southern blots. The HindIII satellite DNA family is conserved in three genera of the Cyprinidae. Variation in nucleotide sequences of this repetitive fragment, which is A+T-rich, is distributed both within individuals and among populations. High overall nucleotide divergence (d(ij) = 0.056 ± 0.001) was detected among clones of the HindIII satellite DNAs of Acrossocheilus paradoxus. Based on the molecular clock hypothesis, the maximum evolutionary rate was estimated to be 5.3 x 10-7 substitutions per site per year. Lineage sorting may have contributed to the genetic heterogeneity within individuals and populations. Cladistic analyses indicated a closer phylogeographic relationship between populations of the central and south regions in Taiwan.

Original languageEnglish
Pages (from-to)780-788
Number of pages9
JournalGenome
Volume42
Issue number4
Publication statusPublished - 1999 Aug 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics

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