Cloning and nucleotide sequence of carbaryl hydrolase gene (cahA) from Arthrobacter sp. RC100

Masayuki Hashimoto, Atsushi Mizutani, Kanako Tago, Mayumi Ohnishi-Kameyama, Takahiro Shimojo, Masahito Hayatsu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A carbaryl hydrolase gene (cahA) encoded on the plasmid pRC1 in Arthrobacter sp. RC100 was cloned and sequenced. The entire region of the deduced amino acid sequence was found to be homologous to that of an amidase family. Parts of the consensus sequences of the amidase gene have been identified in CahA from strain RC100. CahA was overexpressed in Escherichia coli JM109, and the enzyme was purified to homogeneity by protamine sulfate treatment, ammonium sulfate precipitation, and hydrophobic and anion-exchange chromatographies. The purified enzyme showed hydrolase activity toward 1-naphthylacetamide and isobutyramide but showed no activity toward 1-naphthylacetate. This is the first report of an amidase that is able to hydrolyze N-methylcarbamate pesticides.

Original languageEnglish
Pages (from-to)410-414
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume101
Issue number5
DOIs
Publication statusPublished - 2006 May 1

Fingerprint

amidase
Carbaryl
Hydrolases
Cloning
Nucleotides
Organism Cloning
Enzymes
Genes
Pesticides
Chromatography
Escherichia coli
Arthrobacter
Amino acids
Ion exchange
Protamines
Negative ions
Consensus Sequence
Ammonium Sulfate
Anions
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Hashimoto, Masayuki ; Mizutani, Atsushi ; Tago, Kanako ; Ohnishi-Kameyama, Mayumi ; Shimojo, Takahiro ; Hayatsu, Masahito. / Cloning and nucleotide sequence of carbaryl hydrolase gene (cahA) from Arthrobacter sp. RC100. In: Journal of Bioscience and Bioengineering. 2006 ; Vol. 101, No. 5. pp. 410-414.
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Cloning and nucleotide sequence of carbaryl hydrolase gene (cahA) from Arthrobacter sp. RC100. / Hashimoto, Masayuki; Mizutani, Atsushi; Tago, Kanako; Ohnishi-Kameyama, Mayumi; Shimojo, Takahiro; Hayatsu, Masahito.

In: Journal of Bioscience and Bioengineering, Vol. 101, No. 5, 01.05.2006, p. 410-414.

Research output: Contribution to journalArticle

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