Cloning and sequence analysis of the gene (epr Al) encoding an extracellular protease from Aeromonas hydrophila

Tsong Min Chang, Ching-Chuan Liu, Ming Chung Chang

Research output: Contribution to journalArticle

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Abstract

A gene (epr Al) encoding the extracellular protease of Aeromonas hydrophila AHl has been cloned and sequenced. Nucleotide sequence analysis of epr Al predicted a single open reading frame (ORF) of 1,038 bp encoding a 346 amino acid (aa) polypeptide, with a potential 21-aa signal peptide. When the epr AI gene was expressed in minicells, one major band of approx. 37 kDa was identified, while protease activity staining experiments identified a caseinolytic band of approx. 29 kDa determined by SDS-PAGE analysis of the minicells. The deduced C-terminal aa region (Arg-290 to Gly-313) showed sequence homology to partial C-terminal sequences of other zinc metalloproteases including Penicillium citrinum metalloprotease (PlnC), Aspergillus oryzae metalloprotease (NpII), Aspergillus flavus metalloprotease (MepA), and Aspergillus fumigatus metalloprotease (Mep20), particularly with respect to zinc-binding residues.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalGene
Volume199
Issue number1-2
DOIs
Publication statusPublished - 1997 Oct 15

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Aeromonas hydrophila
Metalloproteases
Sequence Analysis
Organism Cloning
Peptide Hydrolases
Genes
Amino Acids
Zinc
Aspergillus oryzae
Aspergillus flavus
Aspergillus fumigatus
Penicillium
Sequence Homology
Protein Sorting Signals
Open Reading Frames
Polyacrylamide Gel Electrophoresis
Staining and Labeling
Peptides

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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title = "Cloning and sequence analysis of the gene (epr Al) encoding an extracellular protease from Aeromonas hydrophila",
abstract = "A gene (epr Al) encoding the extracellular protease of Aeromonas hydrophila AHl has been cloned and sequenced. Nucleotide sequence analysis of epr Al predicted a single open reading frame (ORF) of 1,038 bp encoding a 346 amino acid (aa) polypeptide, with a potential 21-aa signal peptide. When the epr AI gene was expressed in minicells, one major band of approx. 37 kDa was identified, while protease activity staining experiments identified a caseinolytic band of approx. 29 kDa determined by SDS-PAGE analysis of the minicells. The deduced C-terminal aa region (Arg-290 to Gly-313) showed sequence homology to partial C-terminal sequences of other zinc metalloproteases including Penicillium citrinum metalloprotease (PlnC), Aspergillus oryzae metalloprotease (NpII), Aspergillus flavus metalloprotease (MepA), and Aspergillus fumigatus metalloprotease (Mep20), particularly with respect to zinc-binding residues.",
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Cloning and sequence analysis of the gene (epr Al) encoding an extracellular protease from Aeromonas hydrophila. / Chang, Tsong Min; Liu, Ching-Chuan; Chang, Ming Chung.

In: Gene, Vol. 199, No. 1-2, 15.10.1997, p. 225-229.

Research output: Contribution to journalArticle

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