Cloning of an orange-spotted grouper (Epinephelus coioides) Mx cDNA and characterisation of its expression in response to nodavirus

Young Mao Chen, Yong Lin Su, John Han You Lin, Huey Lang Yang, Tzong Yueh Chen

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

Molecular cloning and nucleotide sequencing of cDNA encoding an orange-spotted grouper (Epinephelus coioides) homolog of Mx ("OsgMx") was conducted and its possible role in fish immunity was analysed. Similar to mammalian Mx, the OsgMx are members of a family of interferon-inducible genes that are expressed by cells in response to nodavirus and iridovirus naturally-infected. Expression of OsgMx mRNA was noticeably upregulated in all tissues by nodavirus naturally-infected grouper. The transcription of OsgMx gene increased 6 h after intramuscular injection of nodavirus experimentally- infected fish and peaked at 72 h in their brains. Analysis of the 5′-flanking sequence of the gene shows that as in pufferfish and zebrafish, the OsgMx promoter contains two potential interferon-stimulated response element (ISRE) responsible for the induction of interferon-inducer polyinosinic-polycytidylic acid (Poly[I:C]). Transient transfection of grouper cells in gfp-reporter gene assays shows that the activation of the grouper Mx promoter fragment by Poly[I:C] is sufficient to allow the expression of green fluorescent protein (GFP). These results may provide a possible regulated pathway against nodavirus.

Original languageEnglish
Pages (from-to)58-71
Number of pages14
JournalFish and Shellfish Immunology
Volume20
Issue number1
DOIs
Publication statusPublished - 2006 Jan

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

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