Cloning of mercury resistance determinants in Escherichia coli and analysis of mercury reduction activity in vivo and in vitro

Jo Shu Chang; Yun Peng Chao; Yin Ming Fong; Yuh Ping Hwang; Ping Jei Lin

Cloning of mercury resistance determinants in Escherichia coli and analysis of mercury reduction activity in vivo and in vitro

Jo Shu Chang, Yun Peng Chao, Yin Ming Fong, Yuh Ping Hwang, Ping Jei Lin

Research output: Contribution to journal › Article › peer-review

Abstract

Transposon Tn501-originated mercury resistance determinant (mer operon) located in plasmid pHP45Ω-Hg was subcloned into plasmid pJF118EH, containing the tac promoter, and was subsequently transformed into Escherichia coli VJS632QA. The resulting recombinant strain E. coli PWS1 tolerated Hg²⁺ concentration up to 80 mg/L and exhibited higher specific mercury reduction activity. The expression of mer operon in E. coli PWS1 was affected by inducers IPTG and mercuric ions.

Original language	English
Pages (from-to)	265-274
Number of pages	10
Journal	Journal of the Chinese Institute of Chemical Engineers
Volume	29
Issue number	4
Publication status	Published - 1998 Jul

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering

Cite this

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title = "Cloning of mercury resistance determinants in Escherichia coli and analysis of mercury reduction activity in vivo and in vitro",

abstract = "Transposon Tn501-originated mercury resistance determinant (mer operon) located in plasmid pHP45Ω-Hg was subcloned into plasmid pJF118EH, containing the tac promoter, and was subsequently transformed into Escherichia coli VJS632QA. The resulting recombinant strain E. coli PWS1 tolerated Hg2+ concentration up to 80 mg/L and exhibited higher specific mercury reduction activity. The expression of mer operon in E. coli PWS1 was affected by inducers IPTG and mercuric ions.",

author = "Chang, {Jo Shu} and Chao, {Yun Peng} and Fong, {Yin Ming} and Hwang, {Yuh Ping} and Lin, {Ping Jei}",

year = "1998",

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pages = "265--274",

journal = "Journal of the Chinese Institute of Chemical Engineers",

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T1 - Cloning of mercury resistance determinants in Escherichia coli and analysis of mercury reduction activity in vivo and in vitro

AU - Chang, Jo Shu

AU - Chao, Yun Peng

AU - Fong, Yin Ming

AU - Hwang, Yuh Ping

AU - Lin, Ping Jei

PY - 1998/7

Y1 - 1998/7

N2 - Transposon Tn501-originated mercury resistance determinant (mer operon) located in plasmid pHP45Ω-Hg was subcloned into plasmid pJF118EH, containing the tac promoter, and was subsequently transformed into Escherichia coli VJS632QA. The resulting recombinant strain E. coli PWS1 tolerated Hg2+ concentration up to 80 mg/L and exhibited higher specific mercury reduction activity. The expression of mer operon in E. coli PWS1 was affected by inducers IPTG and mercuric ions.

AB - Transposon Tn501-originated mercury resistance determinant (mer operon) located in plasmid pHP45Ω-Hg was subcloned into plasmid pJF118EH, containing the tac promoter, and was subsequently transformed into Escherichia coli VJS632QA. The resulting recombinant strain E. coli PWS1 tolerated Hg2+ concentration up to 80 mg/L and exhibited higher specific mercury reduction activity. The expression of mer operon in E. coli PWS1 was affected by inducers IPTG and mercuric ions.

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SN - 0368-1653

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JO - Journal of the Chinese Institute of Chemical Engineers

JF - Journal of the Chinese Institute of Chemical Engineers

IS - 4

ER -

Cloning of mercury resistance determinants in Escherichia coli and analysis of mercury reduction activity in vivo and in vitro

Abstract

All Science Journal Classification (ASJC) codes

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