Cloning of mercury resistance determinants in Escherichia coli and analysis of mercury reduction activity in vivo and in vitro

Jo Shu Chang, Yun Peng Chao, Yin Ming Fong, Yuh Ping Hwang, Ping Jei Lin

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1 Citation (Scopus)

Abstract

Transposon Tn501-originated mercury resistance determinant (mer operon) located in plasmid pHP45Ω-Hg was subcloned into plasmid pJF118EH, containing the tac promoter, and was subsequently transformed into Escherichia coli VJS632QA. The resulting recombinant strain E. coli PWS1 tolerated Hg2+ concentration up to 80 mg/L and exhibited higher specific mercury reduction activity. The expression of mer operon in E. coli PWS1 was affected by inducers IPTG and mercuric ions.

Original languageEnglish
Pages (from-to)265-274
Number of pages10
JournalJournal of the Chinese Institute of Chemical Engineers
Volume29
Issue number4
Publication statusPublished - 1998 Jul

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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