Transposon Tn501-originated mercury resistance determinant (mer operon) located in plasmid pHP45Ω-Hg was subcloned into plasmid pJF118EH, containing the tac promoter, and was subsequently transformed into Escherichia coli VJS632QA. The resulting recombinant strain E. coli PWS1 tolerated Hg2+ concentration up to 80 mg/L and exhibited higher specific mercury reduction activity. The expression of mer operon in E. coli PWS1 was affected by inducers IPTG and mercuric ions.
|Number of pages||10|
|Journal||Journal of the Chinese Institute of Chemical Engineers|
|Publication status||Published - 1998 Jul|
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)