Clulture and diferentiation of amniotic stem cells in a microfluidic system

Huei Wen Wu, Xi Zhang Lin, Shiaw Min Hwang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this study, a new microfluidic system is presented which can culture and differentiate MSCs in situ. It is composed of several micro-components, including seeding reservoirs, culture areas, micropumps, microgates, waste reservoirs and fluid microchannels. All of them are fabricated by using micro-electro-mechanical-systems (MEMS) technology. The automated system allows for the long-term culture and differentiation of MSCs. Two methods, including Oil Red O staining for adipogenic cells, and alkaline phosphatase staining for osteogenic cells are used to assess the differentiation of MSCs. Experimental results clearly demonstrate that the MSCs can be cultured for proliferation and different types of differentiation are possible in this microfluidic system.

Original languageEnglish
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages2381-2384
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 11
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: 2009 Jun 212009 Jun 25

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period09-06-2109-06-25

Fingerprint

Stem cells
Microfluidics
Phosphatases
Microchannels
Fluids
Oils

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Wu, H. W., Lin, X. Z., Hwang, S. M., & Lee, G. B. (2009). Clulture and diferentiation of amniotic stem cells in a microfluidic system. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 2381-2384). [5285452] (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285452
Wu, Huei Wen ; Lin, Xi Zhang ; Hwang, Shiaw Min ; Lee, Gwo Bin. / Clulture and diferentiation of amniotic stem cells in a microfluidic system. TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. pp. 2381-2384 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).
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abstract = "In this study, a new microfluidic system is presented which can culture and differentiate MSCs in situ. It is composed of several micro-components, including seeding reservoirs, culture areas, micropumps, microgates, waste reservoirs and fluid microchannels. All of them are fabricated by using micro-electro-mechanical-systems (MEMS) technology. The automated system allows for the long-term culture and differentiation of MSCs. Two methods, including Oil Red O staining for adipogenic cells, and alkaline phosphatase staining for osteogenic cells are used to assess the differentiation of MSCs. Experimental results clearly demonstrate that the MSCs can be cultured for proliferation and different types of differentiation are possible in this microfluidic system.",
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Wu, HW, Lin, XZ, Hwang, SM & Lee, GB 2009, Clulture and diferentiation of amniotic stem cells in a microfluidic system. in TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems., 5285452, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 2381-2384, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, CO, United States, 09-06-21. https://doi.org/10.1109/SENSOR.2009.5285452

Clulture and diferentiation of amniotic stem cells in a microfluidic system. / Wu, Huei Wen; Lin, Xi Zhang; Hwang, Shiaw Min; Lee, Gwo Bin.

TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 2381-2384 5285452 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Wu HW, Lin XZ, Hwang SM, Lee GB. Clulture and diferentiation of amniotic stem cells in a microfluidic system. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 2381-2384. 5285452. (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285452