Clulture and diferentiation of amniotic stem cells in a microfluidic system

Huei Wen Wu, Xi Zhang Lin, Shiaw Min Hwang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this study, a new microfluidic system is presented which can culture and differentiate MSCs in situ. It is composed of several micro-components, including seeding reservoirs, culture areas, micropumps, microgates, waste reservoirs and fluid microchannels. All of them are fabricated by using micro-electro-mechanical-systems (MEMS) technology. The automated system allows for the long-term culture and differentiation of MSCs. Two methods, including Oil Red O staining for adipogenic cells, and alkaline phosphatase staining for osteogenic cells are used to assess the differentiation of MSCs. Experimental results clearly demonstrate that the MSCs can be cultured for proliferation and different types of differentiation are possible in this microfluidic system.

Original languageEnglish
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages2381-2384
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 11
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: 2009 Jun 212009 Jun 25

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period09-06-2109-06-25

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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