Clustered regularly interspaced short palindromic repeats are emm type-specific in highly prevalent group A streptococci

Po Xing Zheng, Yuen Chi Chan, Chien Shun Chiou, Chuan Chiang-Ni, Shu Ying Wang, Pei Jane Tsai, Woei Jer Chuang, Yee Shin Lin, Ching Chuan Liu, Jiunn Jong Wu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) are the bacterial adaptive immune system against foreign nucleic acids. Given the variable nature of CRISPR, it could be a good marker for molecular epidemiology. Group A streptococcus is one of the major human pathogens. It has two CRISPR loci, including CRISPR01 and CRISPR02. The aim of this study was to analyze the distribution of CRISPR-associated gene cassettes (cas) and CRISPR arrays in highly prevalent emm types. The cas cassette and CRISPR array in two CRISPR loci were analyzed in a total of 332 strains, including emm1, emm3, emm4, emm12, and emm28 strains. The CRISPR type was defined by the spacer content of each CRISPR array. All strains had at least one cas cassette or CRISPR array. More than 90% of the spacers were found in one emm type, specifically. Comparing the consistency between emm and CRISPR types by Simpson's index of diversity and the adjusted Wallace coefficient, CRISPR01 type was concordant to emm type, and CRISPR02 showed unidirectional congruence to emm type, suggesting that at least for the majority of isolates causing infection in high income countries, the emm type can be inferred from CRISPR analysis, which can further discriminate isolates sharing the same emm type.

Original languageEnglish
Article numbere0145223
JournalPloS one
Volume10
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

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Clustered Regularly Interspaced Short Palindromic Repeats
Streptococcus
molecular epidemiology
loci
nucleic acids
income
pathogens
infection
genes
Molecular Epidemiology
Immune system
Pathogens

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

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title = "Clustered regularly interspaced short palindromic repeats are emm type-specific in highly prevalent group A streptococci",
abstract = "Clustered regularly interspaced short palindromic repeats (CRISPR) are the bacterial adaptive immune system against foreign nucleic acids. Given the variable nature of CRISPR, it could be a good marker for molecular epidemiology. Group A streptococcus is one of the major human pathogens. It has two CRISPR loci, including CRISPR01 and CRISPR02. The aim of this study was to analyze the distribution of CRISPR-associated gene cassettes (cas) and CRISPR arrays in highly prevalent emm types. The cas cassette and CRISPR array in two CRISPR loci were analyzed in a total of 332 strains, including emm1, emm3, emm4, emm12, and emm28 strains. The CRISPR type was defined by the spacer content of each CRISPR array. All strains had at least one cas cassette or CRISPR array. More than 90{\%} of the spacers were found in one emm type, specifically. Comparing the consistency between emm and CRISPR types by Simpson's index of diversity and the adjusted Wallace coefficient, CRISPR01 type was concordant to emm type, and CRISPR02 showed unidirectional congruence to emm type, suggesting that at least for the majority of isolates causing infection in high income countries, the emm type can be inferred from CRISPR analysis, which can further discriminate isolates sharing the same emm type.",
author = "Zheng, {Po Xing} and Chan, {Yuen Chi} and Chiou, {Chien Shun} and Chuan Chiang-Ni and Wang, {Shu Ying} and Tsai, {Pei Jane} and Chuang, {Woei Jer} and Lin, {Yee Shin} and Liu, {Ching Chuan} and Wu, {Jiunn Jong}",
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Clustered regularly interspaced short palindromic repeats are emm type-specific in highly prevalent group A streptococci. / Zheng, Po Xing; Chan, Yuen Chi; Chiou, Chien Shun; Chiang-Ni, Chuan; Wang, Shu Ying; Tsai, Pei Jane; Chuang, Woei Jer; Lin, Yee Shin; Liu, Ching Chuan; Wu, Jiunn Jong.

In: PloS one, Vol. 10, No. 12, e0145223, 01.12.2015.

Research output: Contribution to journalArticle

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AU - Zheng, Po Xing

AU - Chan, Yuen Chi

AU - Chiou, Chien Shun

AU - Chiang-Ni, Chuan

AU - Wang, Shu Ying

AU - Tsai, Pei Jane

AU - Chuang, Woei Jer

AU - Lin, Yee Shin

AU - Liu, Ching Chuan

AU - Wu, Jiunn Jong

PY - 2015/12/1

Y1 - 2015/12/1

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