Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model

Pao Yen Lin, Yeng Ting Wu, Guan Cheng Lin, Yao Hsiang Shih, Ariunaa Sampilvanjil, Lih Ren Chen, Yu Jen Yang, Hua Lin Wu, Meei Jyh Jiang

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Objective: Hemodynamic stress participates in the initiation and progression of aneurysmal degeneration. Coarctation increases flow-mediated stress on the aortic wall. We tested the hypothesis that prolonged coarctation of an infrarenal abdominal aorta (AA) segment leads to abdominal aortic aneurysm (AAA) formation in mini pigs. Methods: An asymmetric, funnel-shaped flow path was created by constricting the infrarenal AA segment of Taiwanese Lanyu mini pigs (age, 7-10 months; male and female) wrapped with an 8-mm-wide expanded polytetrafluoroethylene Teflon strip for 4 weeks (4w), 8 weeks (8w), and 12 weeks (12w) (seven pigs per group). This mimics the tortuous aneurysm neck in human AAA, which increases downstream flow-mediated stress. Significant flow disturbance resulting from moderate coarctation was indicated by a pulsatility index reduced to one third the inherent levels. Sham control pigs received Teflon wrapping without coarctation. Results: Aneurysm characterized by progressive medial degeneration occurred at the terminal AA after 12w coarctation. The outer dimension enlargement of the distal AA exceeded 50% compared with that of the proximal AA at 4w, 8w, and 12w postcoarctation (sham, 1.0; 4w, 1.7 ± 0.08; 8w, 1.5 ± 0.09; 12w, 1.7 ± 0.01). Lumen ratio of the distal-to-suprarenal AA increased time dependently, with 12w postcoarctation exhibiting significant increase (sham, 1.0 ± 0.05; 4w, 1.1 ± 0.11; 8w, 1.4 ± 0.20; 12w, 1.5 ± 0.09). In the distal AA, elastic lamellae exhibited fragmentation at 4w and more pronounced fragmentation with decreased density at 8w and 12w postcoarctation. Medial collagen density exhibited the trend to increase at 4w and 8w but was reversed at 12w postcoarctation. Smooth muscle exhibited disarray and nuclear density decrease at 8w and 12w postcoarctation (sham, 6966 ± 888/mm2; 4w, 5747 ± 1340/mm2; 8w, 4153 ± 323/mm2; 12w, 4083 ± 465/mm2). Gelatin zymography revealed that matrix metalloproteinase-9 activity markedly increased at 4w postcoarctation. Conclusions: Prolonged moderate coarctation caused regional hemodynamic stress and thereby induced degenerative AAA in the terminal AA.

Original languageEnglish
Pages (from-to)806-815.e1
JournalJournal of Vascular Surgery
Volume57
Issue number3
DOIs
Publication statusPublished - 2013 Mar

All Science Journal Classification (ASJC) codes

  • Surgery
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model'. Together they form a unique fingerprint.

Cite this