Codon usage regulates protein structure and function by affecting translation elongation speed in Drosophila cells

Fangzhou Zhao, Chien Hung Yu, Yi Liu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Codon usage biases are found in all eukaryotic and prokaryotic genomes and have been proposed to regulate different aspects of translation process. Codon optimality has been shown to regulate translation elongation speed in fungal systems, but its effect on translation elongation speed in animal systems is not clear. In this study, we used a Drosophila cell-free translation system to directly compare the velocity of mRNA translation elongation. Our results demonstrate that optimal synonymous codons speed up translation elongation while non-optimal codons slow down translation. In addition, codon usage regulates ribosome movement and stalling on mRNA during translation. Finally, we show that codon usage affects protein structure and function in vitro and in Drosophila cells. Together, these results suggest that the effect of codon usage on translation elongation speed is a conserved mechanism from fungi to animals that can affect protein folding in eukaryotic organisms.

Original languageEnglish
Pages (from-to)8484-8492
Number of pages9
JournalNucleic acids research
Volume45
Issue number14
DOIs
Publication statusPublished - 2017 Jan 1

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Codon
Drosophila
Proteins
Protein Biosynthesis
Cell-Free System
Protein Folding
Ribosomes
Fungi
Genome

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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Codon usage regulates protein structure and function by affecting translation elongation speed in Drosophila cells. / Zhao, Fangzhou; Yu, Chien Hung; Liu, Yi.

In: Nucleic acids research, Vol. 45, No. 14, 01.01.2017, p. 8484-8492.

Research output: Contribution to journalArticle

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