TY - JOUR
T1 - Collagenolytic activity of crustacean midgut serine proteases
T2 - Comparison with the bacterial and mammalian enzymes
AU - Yuh-Ling Chen, Chen
AU - Pei-Jung Lu, Lu
AU - Inn-Ho Tsai, Tsai
N1 - Funding Information:
study was supported by National Science Council, Taiwan, Republic of China.
PY - 1991
Y1 - 1991
N2 - 1. 1. We have investigated the collagenolytic activity of the following serine proteases: proteinase K, subtilisin Novo, Staphylococcal endoproteinase Glu-C, Streptomyces pronases, the trypsins and chymotrypsins from shrimp midgut and bovine pancreas. 2. 2. By assays on both the insoluble 3H-collagen fibrils and the soluble type I collagen, it was demonstrated that the shrimp midgut serine proteases, and less efficiently, the pronases from Streptomyces griseus, could hydrolyze collagen while the other serine proteases tested could not. 3. 3. Our data indicate that the trypsins and chymotrypsins of shrimp (Penaeus monodon) directly and indirectly digest native collagen, and that the indirect pathway probably involves activation of procollagenase in the native collagen by these serine proteases.
AB - 1. 1. We have investigated the collagenolytic activity of the following serine proteases: proteinase K, subtilisin Novo, Staphylococcal endoproteinase Glu-C, Streptomyces pronases, the trypsins and chymotrypsins from shrimp midgut and bovine pancreas. 2. 2. By assays on both the insoluble 3H-collagen fibrils and the soluble type I collagen, it was demonstrated that the shrimp midgut serine proteases, and less efficiently, the pronases from Streptomyces griseus, could hydrolyze collagen while the other serine proteases tested could not. 3. 3. Our data indicate that the trypsins and chymotrypsins of shrimp (Penaeus monodon) directly and indirectly digest native collagen, and that the indirect pathway probably involves activation of procollagenase in the native collagen by these serine proteases.
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U2 - 10.1016/0305-0491(91)90287-N
DO - 10.1016/0305-0491(91)90287-N
M3 - Article
C2 - 1782759
AN - SCOPUS:0026341149
SN - 0305-0491
VL - 100
SP - 763
EP - 768
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
IS - 4
ER -