Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells

Jin Yuan Shih, Shuenn Chen Yang, Tse-Ming Hong, Ang Yuan, Jeremy J.W. Chen, Chong Jen Yu, Yih Leong Chang, Yung Chie Lee, Konan Peck, Cheng Wen Wu, Pan Chyr Yang

Research output: Contribution to journalArticle

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Abstract

Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.

Original languageEnglish
Pages (from-to)1392-1400
Number of pages9
JournalJournal of the National Cancer Institute
Volume93
Issue number18
DOIs
Publication statusPublished - 2001 Sep 19

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Neoplasm Metastasis
Neoplasms
Lung Neoplasms
Genes
Northern Blotting
collapsin response mediator protein-1
Reverse Transcription
Western Blotting
Telophase
Suppressor Genes
Polymerase Chain Reaction
Centrosome
Spindle Apparatus
Pseudopodia
Interphase
Fluorescence Microscopy
Mitosis
Cytoplasm
Lymph Nodes
Observation

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Shih, Jin Yuan ; Yang, Shuenn Chen ; Hong, Tse-Ming ; Yuan, Ang ; Chen, Jeremy J.W. ; Yu, Chong Jen ; Chang, Yih Leong ; Lee, Yung Chie ; Peck, Konan ; Wu, Cheng Wen ; Yang, Pan Chyr. / Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells. In: Journal of the National Cancer Institute. 2001 ; Vol. 93, No. 18. pp. 1392-1400.
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title = "Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells",
abstract = "Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.",
author = "Shih, {Jin Yuan} and Yang, {Shuenn Chen} and Tse-Ming Hong and Ang Yuan and Chen, {Jeremy J.W.} and Yu, {Chong Jen} and Chang, {Yih Leong} and Lee, {Yung Chie} and Konan Peck and Wu, {Cheng Wen} and Yang, {Pan Chyr}",
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Shih, JY, Yang, SC, Hong, T-M, Yuan, A, Chen, JJW, Yu, CJ, Chang, YL, Lee, YC, Peck, K, Wu, CW & Yang, PC 2001, 'Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells', Journal of the National Cancer Institute, vol. 93, no. 18, pp. 1392-1400. https://doi.org/10.1093/jnci/93.18.1392

Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells. / Shih, Jin Yuan; Yang, Shuenn Chen; Hong, Tse-Ming; Yuan, Ang; Chen, Jeremy J.W.; Yu, Chong Jen; Chang, Yih Leong; Lee, Yung Chie; Peck, Konan; Wu, Cheng Wen; Yang, Pan Chyr.

In: Journal of the National Cancer Institute, Vol. 93, No. 18, 19.09.2001, p. 1392-1400.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells

AU - Shih, Jin Yuan

AU - Yang, Shuenn Chen

AU - Hong, Tse-Ming

AU - Yuan, Ang

AU - Chen, Jeremy J.W.

AU - Yu, Chong Jen

AU - Chang, Yih Leong

AU - Lee, Yung Chie

AU - Peck, Konan

AU - Wu, Cheng Wen

AU - Yang, Pan Chyr

PY - 2001/9/19

Y1 - 2001/9/19

N2 - Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.

AB - Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.

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DO - 10.1093/jnci/93.18.1392

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JO - Journal of the National Cancer Institute

JF - Journal of the National Cancer Institute

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