Combination of iron-silicate adsorption and Thermosynechococcus sp. CL-1 cultivation for swine wastewater treatment, CO2 fixation, phycobiliproteins generation

Birgitta Narindri Rara Winayu, Da Siou Chen, Hsin Ta Hsueh, Hong Ping Lin, Hsin Chu

Research output: Contribution to journalArticlepeer-review


Implementation of photosynthetic microorganism, like Thermosynechococcus sp. CL-1 (TCL-1), is considered as an eco-friendly strategy for carbon dioxide fixation and nutrient utilization in wastewater. However, high level of ammoniacal nitrogen carries out toxicity to TCL-1 cell and adsorption can be applied as a pre-treatment stage. This study observed application of iron-silicate on ammoniacal nitrogen adsorption in non-sterilized and undiluted swine wastewater as pre-treatment for TCL-1 cultivation for CO2 fixation, NH4+-N degradation, and phycobiliprotein generation. The utilization of 100 g/L iron-silicate performed efficient adsorption with the quantity of 4.05 mg-N/g-Fe/Si and fitted the pseudo-first-order adsorption with R2 value of 0.993. The adsorption pre-treated swine wastewater was implemented as TCL-1 growth medium under the 1000 μE/m2/s light intensity for 12 h. The TCL-1 biomass growth, CO2 fixation, and NH4+-N removal rate during cultivation period reached to 31.67 ± 1.25; 57.54 ± 6.09; and 6.05 ± 0.20 mg/L/h respectively. Phycobiliprotein as a byproduct was generated with the productivity of 13.9 mg/g/h. Thus, coupling iron-silicate for pre-treatment step of TCL-1 cultivation in swine wastewater is eco-friendly strategy to reduce water demand for dilution, ignite phycobiliprotein accumulation during the CO2 fixation and nutrient utilization process.

Original languageEnglish
Article number102406
JournalJournal of Water Process Engineering
Publication statusPublished - 2021 Dec

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Safety, Risk, Reliability and Quality
  • Waste Management and Disposal
  • Process Chemistry and Technology


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