Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat

Kuan Hung Chen, Chien Hui Yang, Christopher Glenn Wallace, Chung Ren Lin, Chia Kai Liu, Tsung Cheng Yin, Tien Hung Huang, Yi Ling Chen, Cheuk Kwan Sun, Hon Kan Yip

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

This study tested the hypothesis that combination therapy using extracorporeal shock wave (ECSW)-mela-tonin (Mel) was superior to either alone at ameliorating neuropathic pain (NP). NP was induced by chronic constriction injury (CCI) to the left sciatic nerve in rats. Animals were categorized into sham control (group 1), CCI only (group 2), CCI-ECSW (group 3), CCI-Mel (group 4) and CCI-ECSW-Mel (group 5). By days 2 and 8 after CCI, the mechanical paw withdrawal threshold (MPWT)/thermal paw withdrawal latency (TPWL) were highest in group 2, lowest in group 1, significantly lower in group 5 than in groups 3 and 4 (all p<0.0001), and not significantly different between groups 3 and 4. The protein expressions of inflammatory (TNF-α/NF-κB/MMP-9/IL-1ß/GFAP/ox42), oxidative-stress (NOX-1/NOX-2/NOX-4/oxidized protein), DNA/mitochondrial-damaged (γ-H2AX/cytosolic mitochondria), apoptotic (cleaved capase-3/PARP), and MAPK family biomarkers (p-P38/p-JNK/p-ERK1/2) in dorsal root ganglia and spinal dorsal horn expressed a similar pattern of MPWT/TPWL among the five groups, except for significantly higher in group 4 than in group 3 (all p<0.0001). The protein expressions of Nav.1.3, Nav.1.8 and Nav.1.9 in sciatic nerve displayed an identical pattern to inflammation among the five groups (all p<0.001). Pain facilitated cellular expressions (p-P38+/peripherin+ cells, P38+/NF200+ cells) displayed an identical pattern to inflammation among the five groups (all p<0.0001). In conclusion, ECSW-Mel combination therapy markedly ameliorated NP induced by CCI.

Original languageEnglish
Article numberAJTR0057270
Pages (from-to)4593-4606
Number of pages14
JournalAmerican Journal of Translational Research
Volume9
Issue number10
Publication statusPublished - 2017

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Clinical Biochemistry
  • Cancer Research

Fingerprint

Dive into the research topics of 'Combination therapy with extracorporeal shock wave and melatonin markedly attenuated neuropathic pain in rat'. Together they form a unique fingerprint.

Cite this