Combining engineering strategies and fermentation technology to enhance docosahexaenoic acid (DHA) production from an indigenous Thraustochytrium sp. BM2 strain

Chun Yen Chen, Ya Ting Yang

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The DHA productivity of Thraustochytrium sp. BM2 was improved by optimizing the bioreactor operation conditions. The effects of agitation rate and aeration rate on the microalgal growth and DHA production were first studied in 5-L stirred-tank fermenter. The results showed that 100 rpm agitation and 1.2 vvm aeration (i.e., kLa = 27 ± 1.1 h−1) achieved the highest biomass (4590 mg/L/d) and DHA (379 mg/L/d) productivities. Next, using an optimal glucose concentration (60 g/L) led to the highest biomass (5580 mg/L/d) and DHA (457 mg/L/d) productivities. The cellular DHA content decreased when increasing the agitation rate, probably due to the sensitivity of this strain to the shear stress. The effect of pneumatic and mechanical agitation under the same kLa was studied with two different types of fermenters (stirred-tank and air-lift) and cultivation in air-lift fermenter resulted in better biomass production (27.57 g/L), cellular DHA content (13.1%) and DHA productivity (610 mg/L/d).

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalBiochemical Engineering Journal
Volume133
DOIs
Publication statusPublished - 2018 May 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Combining engineering strategies and fermentation technology to enhance docosahexaenoic acid (DHA) production from an indigenous Thraustochytrium sp. BM2 strain'. Together they form a unique fingerprint.

Cite this