Combining enzymatic hydrolysis and dark-photo fermentation processes for hydrogen production from starch feedstock: A feasibility study

Yung Chung Lo, Shing Der Chen, Chun Yen Chen, Tien I. Huang, Chiu Yue Lin, Jo Shu Chang

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In this work, an integrated enzymatic hydrolysis and dark-photo fermentation were employed to enhance the performance of H 2 production from starch feedstock. The starch feedstock was first hydrolyzed in sequencing batch reactor containing indigenous starch hydrolytic bacterium Caldimonas taiwanensis On1, producing reducing sugar at a yield and rate of 0.5 g reducing sugar/g starch and 1.17 g reducing sugar/h/L, respectively, under the optimal condition of pH 7.0, 55 °C and 1.0 vvm (air volume per reactor volume per minute) aeration rate. The hydrolyzed starch was continuously introduced to dark fermentation bioreactor, where the hydrolysate was converted to H 2 at a rate of 0.22 L/h/L by Clostridium butyricum CGS2 at pH 5.8-6.0, 37 °C and 12 h HRT. The resulting effluent from dark fermentation became the influent of continuous photo H 2 production process inoculated with Rhodopseudomonas palustris WP3-5 under the condition of 35 °C, 100 W/m 2 irradiation, pH 7.0 and 48 h HRT. Combining enzymatic hydrolysis, dark fermentation and photo fermentation led to a marked improvement of overall H 2 yield (up to 16.1 mmol H 2 /g COD or 3.09 mol H 2 /mol glucose) and COD removal efficiency (ca. 54.3%), suggesting the potential of using the proposed integrated process for efficient and high-yield bioH 2 production from starch feedstock.

Original languageEnglish
Pages (from-to)5224-5233
Number of pages10
JournalInternational Journal of Hydrogen Energy
Volume33
Issue number19
DOIs
Publication statusPublished - 2008 Oct 1

Fingerprint

fermentation
Enzymatic hydrolysis
starches
hydrogen production
Hydrogen production
Starch
Fermentation
Feedstocks
hydrolysis
sugars
Sugars
crack opening displacement
reactors
Clostridium
aeration
bioreactors
sequencing
effluents
Batch reactors
Bioreactors

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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abstract = "In this work, an integrated enzymatic hydrolysis and dark-photo fermentation were employed to enhance the performance of H 2 production from starch feedstock. The starch feedstock was first hydrolyzed in sequencing batch reactor containing indigenous starch hydrolytic bacterium Caldimonas taiwanensis On1, producing reducing sugar at a yield and rate of 0.5 g reducing sugar/g starch and 1.17 g reducing sugar/h/L, respectively, under the optimal condition of pH 7.0, 55 °C and 1.0 vvm (air volume per reactor volume per minute) aeration rate. The hydrolyzed starch was continuously introduced to dark fermentation bioreactor, where the hydrolysate was converted to H 2 at a rate of 0.22 L/h/L by Clostridium butyricum CGS2 at pH 5.8-6.0, 37 °C and 12 h HRT. The resulting effluent from dark fermentation became the influent of continuous photo H 2 production process inoculated with Rhodopseudomonas palustris WP3-5 under the condition of 35 °C, 100 W/m 2 irradiation, pH 7.0 and 48 h HRT. Combining enzymatic hydrolysis, dark fermentation and photo fermentation led to a marked improvement of overall H 2 yield (up to 16.1 mmol H 2 /g COD or 3.09 mol H 2 /mol glucose) and COD removal efficiency (ca. 54.3{\%}), suggesting the potential of using the proposed integrated process for efficient and high-yield bioH 2 production from starch feedstock.",
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Combining enzymatic hydrolysis and dark-photo fermentation processes for hydrogen production from starch feedstock : A feasibility study. / Lo, Yung Chung; Chen, Shing Der; Chen, Chun Yen; Huang, Tien I.; Lin, Chiu Yue; Chang, Jo Shu.

In: International Journal of Hydrogen Energy, Vol. 33, No. 19, 01.10.2008, p. 5224-5233.

Research output: Contribution to journalArticle

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AU - Lo, Yung Chung

AU - Chen, Shing Der

AU - Chen, Chun Yen

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AU - Chang, Jo Shu

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