Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis

Chi Chang Lin, Yi Heng Ho, Ying Mei Yang, Hsien Chang Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori #603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70% and 80% of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.

Original languageEnglish
Title of host publication3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Pages366-369
Number of pages4
DOIs
Publication statusPublished - 2008 Sep 1
Event3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China
Duration: 2008 Jan 62008 Jan 9

Publication series

Name3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

Other

Other3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
CountryChina
CitySanya
Period08-01-0608-01-09

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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