Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis

Chi Chang Lin, Yi Heng Ho, Ying Mei Yang, Hsien-Chang Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori #603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70% and 80% of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.

Original languageEnglish
Title of host publication3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Pages366-369
Number of pages4
DOIs
Publication statusPublished - 2008 Sep 1
Event3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China
Duration: 2008 Jan 62008 Jan 9

Publication series

Name3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

Other

Other3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
CountryChina
CitySanya
Period08-01-0608-01-09

Fingerprint

Electrophoresis
Raman scattering
Genes
Electric potential

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Lin, C. C., Ho, Y. H., Yang, Y. M., & Chang, H-C. (2008). Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 (pp. 366-369). [4484353] (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS). https://doi.org/10.1109/NEMS.2008.4484353
Lin, Chi Chang ; Ho, Yi Heng ; Yang, Ying Mei ; Chang, Hsien-Chang. / Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. 2008. pp. 366-369 (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS).
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abstract = "A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori #603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70{\%} and 80{\%} of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.",
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Lin, CC, Ho, YH, Yang, YM & Chang, H-C 2008, Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. in 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008., 4484353, 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS, pp. 366-369, 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008, Sanya, China, 08-01-06. https://doi.org/10.1109/NEMS.2008.4484353

Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. / Lin, Chi Chang; Ho, Yi Heng; Yang, Ying Mei; Chang, Hsien-Chang.

3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. 2008. p. 366-369 4484353 (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lin CC, Ho YH, Yang YM, Chang H-C. Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. 2008. p. 366-369. 4484353. (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS). https://doi.org/10.1109/NEMS.2008.4484353