Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis

Chi Chang Lin; Yi Heng Ho; Ying Mei Yang; Hsien Chang Chang

doi:10.1109/NEMS.2008.4484353

Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis

Chi Chang Lin
, Yi Heng Ho
, Ying Mei Yang
, Hsien Chang Chang

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori #603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70% and 80% of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.

Original language	English
Title of host publication	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Pages	366-369
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2008.4484353
Publication status	Published - 2008 Sept 1
Event	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China Duration: 2008 Jan 6 → 2008 Jan 9

Publication series

Name	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

Other

Other	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Country/Territory	China
City	Sanya
Period	08-01-06 → 08-01-09

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Control and Systems Engineering
Electrical and Electronic Engineering

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10.1109/NEMS.2008.4484353

Cite this

Lin, C. C., Ho, Y. H., Yang, Y. M., & Chang, H. C. (2008). Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 (pp. 366-369). Article 4484353 (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS). https://doi.org/10.1109/NEMS.2008.4484353

Lin, Chi Chang ; Ho, Yi Heng ; Yang, Ying Mei et al. / Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. 2008. pp. 366-369 (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS).

@inproceedings{22fd2594b9264879bd7b441e00fe57aa,

title = "Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis",

abstract = "A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori \#238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori \#603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70\% and 80\% of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.",

author = "Lin, \{Chi Chang\} and Ho, \{Yi Heng\} and Yang, \{Ying Mei\} and Chang, \{Hsien Chang\}",

year = "2008",

month = sep,

day = "1",

doi = "10.1109/NEMS.2008.4484353",

language = "English",

isbn = "9781424419081",

series = "3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS",

pages = "366--369",

booktitle = "3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008",

note = "3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 ; Conference date: 06-01-2008 Through 09-01-2008",

}

Lin, CC, Ho, YH, Yang, YM & Chang, HC 2008, Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. in 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008., 4484353, 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS, pp. 366-369, 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008, Sanya, China, 08-01-06. https://doi.org/10.1109/NEMS.2008.4484353

Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. / Lin, Chi Chang; Ho, Yi Heng; Yang, Ying Mei et al.
3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. 2008. p. 366-369 4484353 (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis

AU - Lin, Chi Chang

AU - Ho, Yi Heng

AU - Yang, Ying Mei

AU - Chang, Hsien Chang

PY - 2008/9/1

Y1 - 2008/9/1

N2 - A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori #603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70% and 80% of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.

AB - A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H. pylori #238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori #603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70% and 80% of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.

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U2 - 10.1109/NEMS.2008.4484353

DO - 10.1109/NEMS.2008.4484353

M3 - Conference contribution

AN - SCOPUS:50249088932

SN - 9781424419081

T3 - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

SP - 366

EP - 369

BT - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008

T2 - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008

Y2 - 6 January 2008 through 9 January 2008

ER -

Lin CC, Ho YH, Yang YM, Chang HC. Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. 2008. p. 366-369. 4484353. (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS). doi: 10.1109/NEMS.2008.4484353

Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis

Abstract

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