Cometabolic degradation kinetics of TCE and phenol by Pseudomonas putida

Yan Min Chen, Tsair-Fuh Lin, Chih Huang, Jui-Che Lin

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Modeling of cometabolic kinetics is important for better understanding of degradation reaction and in situ application of bio-remediation. In this study, a model incorporated cell growth and decay, loss of transformation activity, competitive inhibition between growth substrate and non-growth substrate and self-inhibition of non-growth substrate was proposed to simulate the degradation kinetics of phenol and trichloroethylene (TCE) by Pseudomonas putida. All the intrinsic parameters employed in this study were measured independently, and were then used for predicting the batch experimental data. The model predictions conformed well to the observed data at different phenol and TCE concentrations. At low TCE concentrations (<2 mg l-1), the models with or without self-inhibition of non-growth substrate both simulated the experimental data well. However, at higher TCE concentrations (>6 mg l-1), only the model considering self-inhibition can describe the experimental data, suggesting that a self-inhibition of TCE was present in the system. The proposed model was also employed in predicting the experimental data conducted in a repeated batch reactor, and good agreements were observed between model predictions and experimental data. The results also indicated that the biomass loss in the degradation of TCE below 2 mg l-1 can be totally recovered in the absence of TCE for the next cycle, and it could be used for the next batch experiment for the degradation of phenol and TCE. However, for higher concentration of TCE (>6 mg l-1), the recovery of biomass may not be as good as that at lower TCE concentrations.

Original languageEnglish
Pages (from-to)1671-1680
Number of pages10
JournalChemosphere
Volume72
Issue number11
DOIs
Publication statusPublished - 2008 Aug 1

Fingerprint

Trichloroethylene
Pseudomonas putida
trichloroethylene
Phenol
Phenols
phenol
Degradation
kinetics
degradation
Kinetics
substrate
Biomass
Substrates
Bioremediation
biomass
Batch reactors
Cell growth
Growth
prediction
bioremediation

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Chen, Yan Min ; Lin, Tsair-Fuh ; Huang, Chih ; Lin, Jui-Che. / Cometabolic degradation kinetics of TCE and phenol by Pseudomonas putida. In: Chemosphere. 2008 ; Vol. 72, No. 11. pp. 1671-1680.
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Cometabolic degradation kinetics of TCE and phenol by Pseudomonas putida. / Chen, Yan Min; Lin, Tsair-Fuh; Huang, Chih; Lin, Jui-Che.

In: Chemosphere, Vol. 72, No. 11, 01.08.2008, p. 1671-1680.

Research output: Contribution to journalArticle

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