TY - JOUR
T1 - Comparative analyses of the gene expression profiles of Arabidopsis intact plant and cultured cells
AU - Iwase, Akira
AU - Ishii, Hideki
AU - Aoyagi, Hideki
AU - Ohme-Takagi, Masaru
AU - Tanaka, Hideo
N1 - Funding Information:
The authors thank Drs K. Matsui, T. Koyama and K. Hiratsu (Gene Function Research Center, AIST, Japan) for useful discussion and experimental instruction. This work was supported in part by: (1) the Sasakawa Scientific Research Grant from the Japan Science Society and (2) the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
PY - 2005/8
Y1 - 2005/8
N2 - To characterize de-differentiated plant cultured cells and to determine factor(s) that regulate the de-differentiated state, comparative analyses of the gene expression profiles of intact plants and three different cultured cell lines of Arabidopsis were performed by DNA microarray and RNA blot analyses. The three different cultured cell lines (T87 and two cell lines newly induced from leaf and root tissue, respectively) were used. Twenty-seven to 140 genes were down-regulated but only 9-27 genes were up-regulated in each cultured cell lines when compared to the intact plants. Genes commonly regulated in each cultured cell lines were investigated and six genes, including those related to metabolism, were found to be down-regulated and two genes, that include those related to a transcription factor, were up-regulated.
AB - To characterize de-differentiated plant cultured cells and to determine factor(s) that regulate the de-differentiated state, comparative analyses of the gene expression profiles of intact plants and three different cultured cell lines of Arabidopsis were performed by DNA microarray and RNA blot analyses. The three different cultured cell lines (T87 and two cell lines newly induced from leaf and root tissue, respectively) were used. Twenty-seven to 140 genes were down-regulated but only 9-27 genes were up-regulated in each cultured cell lines when compared to the intact plants. Genes commonly regulated in each cultured cell lines were investigated and six genes, including those related to metabolism, were found to be down-regulated and two genes, that include those related to a transcription factor, were up-regulated.
UR - http://www.scopus.com/inward/record.url?scp=24044547087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24044547087&partnerID=8YFLogxK
U2 - 10.1007/s10529-005-8456-x
DO - 10.1007/s10529-005-8456-x
M3 - Article
C2 - 16132859
AN - SCOPUS:24044547087
SN - 0141-5492
VL - 27
SP - 1097
EP - 1103
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 15
ER -