TY - JOUR
T1 - Comparative Genomic Analysis of a Novel Strain of Taiwan Hot-Spring Cyanobacterium Thermosynechococcus sp. CL-1
AU - Cheng, Yen I.
AU - Chou, Lin
AU - Chiu, Yi Fang
AU - Hsueh, Hsin Ta
AU - Kuo, Chih Horng
AU - Chu, Hsiu An
N1 - Funding Information:
We thank the High-Throughput Sequencing Core in the Biodiversity Research Center at Academia Sinica (Taipei, Taiwan) for the PacBio sequencing service and assistance in genome assembly. We also thank Dr. Wen-Dar Lin in the Bioinformatics Core Facility of the Institute of Plant and Microbial Biology, Academia Sinica, for assistance in comparative genome analysis and Ms. Shu-Jen Chou in the Genomic Technology Core Lab of the Institute of Plant and Microbial Biology for assistance in genomic DNA library preparations. Funding. This funding for this work was provided by the Academia Sinica to C-HK and H-AC, and the Ministry of Science and Technology in Taiwan (MOST 108-2311-B-001-024) to H-AC. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Funding Information:
This funding for this work was provided by the Academia Sinica to C-HK and H-AC, and the Ministry of Science and Technology
Publisher Copyright:
© Copyright © 2020 Cheng, Chou, Chiu, Hsueh, Kuo and Chu.
PY - 2020/1/31
Y1 - 2020/1/31
N2 - Thermosynechococcus is a genus of thermophilic unicellular cyanobacteria that are dominant in microbial mats at about 50–65°C in alkaline hot springs of eastern Asia. We used PacBio SMRT Sequencing to sequence the complete genome of a novel strain of thermophilic cyanobacterium, Thermosynechococcus sp. CL-1, isolated from the Chin-Lun hot spring (pH 9.3, 62°C) in Taiwan. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) results suggested that CL-1 is a new species in the genus Thermosynechococcus. Comparative genome analysis revealed divergent genome structures of Thermosynechococcus strains. In addition, the distinct genetic differences between CL-1 and the other Thermosynechococcus strains are related to photosynthesis, transporters, signal transduction, the chaperone/usher system, nitric oxide protection, antibiotic resistance, prokaryotic immunity systems, and other physiological processes. This study suggests that Thermosynechococcus strains have actively acquired many putative horizontally transferred genes from other bacteria that enabled them to adapt to different ecological niches and stressful conditions in hot springs.
AB - Thermosynechococcus is a genus of thermophilic unicellular cyanobacteria that are dominant in microbial mats at about 50–65°C in alkaline hot springs of eastern Asia. We used PacBio SMRT Sequencing to sequence the complete genome of a novel strain of thermophilic cyanobacterium, Thermosynechococcus sp. CL-1, isolated from the Chin-Lun hot spring (pH 9.3, 62°C) in Taiwan. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) results suggested that CL-1 is a new species in the genus Thermosynechococcus. Comparative genome analysis revealed divergent genome structures of Thermosynechococcus strains. In addition, the distinct genetic differences between CL-1 and the other Thermosynechococcus strains are related to photosynthesis, transporters, signal transduction, the chaperone/usher system, nitric oxide protection, antibiotic resistance, prokaryotic immunity systems, and other physiological processes. This study suggests that Thermosynechococcus strains have actively acquired many putative horizontally transferred genes from other bacteria that enabled them to adapt to different ecological niches and stressful conditions in hot springs.
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U2 - 10.3389/fmicb.2020.00082
DO - 10.3389/fmicb.2020.00082
M3 - Article
AN - SCOPUS:85079501815
SN - 1664-302X
VL - 11
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 82
ER -