Comparative proteomic analysis of peritoneal dialysate from chronic glomerulonephritis patients

Hsin Yi Wu, Alex Chien Hwa Liao, Chien Cheng Huang, Pao-Chi Liao, Chih Chiang Chien, Wei Chih Kan, Hsien Yi Wang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Peritoneal dialysis (PD) frequently contributes to peritoneal damage which cannot be easily identified without invasive techniques, implying the urgent need for biomarkers and revealing mechanisms. Chronic glomerulonephritis (CGN) is one of the leading causes of receiving dialysis treatment. Here, we attempted to analyze the peritoneal dialysate collected from CGN patients when they receive continuous ambulatory peritoneal dialysis (CAPD) treatment for the first time and after a year to reveal the protein changes that resulted from PD. Proteins were displayed by two-dimensional gel electrophoresis (2DE). Altered gel spots were digested followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. Eight proteins were found to have differential expression levels between two groups. Their differential expressions were validated by Western blots in other sets of peritoneal dialysates. Proteins identified with higher levels in the first-time dialysate suggested their dominant appearance in CGN patients, while those that showed higher levels in peritoneal dialysate collected after one year may result from initial peritoneal inflammation or changes in the permeability of the peritoneum to middle-sized proteins. All the identified proteins may provide a perceptiveness of peritoneal changes caused by PD and may function as potential biomarkers or drug targets.

Original languageEnglish
Article number863860
JournalBioMed research international
Volume2013
DOIs
Publication statusPublished - 2013 Jun 12

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Dialysis Solutions
Glomerulonephritis
Proteomics
Dialysis
Peritoneal Dialysis
Proteins
Biomarkers
Gels
Continuous Ambulatory Peritoneal Dialysis
Peritoneum
Liquid chromatography
Electrophoresis, Gel, Two-Dimensional
Tandem Mass Spectrometry
Electrophoresis
Liquid Chromatography
Mass spectrometry
Permeability
Western Blotting
Inflammation
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Wu, Hsin Yi ; Liao, Alex Chien Hwa ; Huang, Chien Cheng ; Liao, Pao-Chi ; Chien, Chih Chiang ; Kan, Wei Chih ; Wang, Hsien Yi. / Comparative proteomic analysis of peritoneal dialysate from chronic glomerulonephritis patients. In: BioMed research international. 2013 ; Vol. 2013.
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Comparative proteomic analysis of peritoneal dialysate from chronic glomerulonephritis patients. / Wu, Hsin Yi; Liao, Alex Chien Hwa; Huang, Chien Cheng; Liao, Pao-Chi; Chien, Chih Chiang; Kan, Wei Chih; Wang, Hsien Yi.

In: BioMed research international, Vol. 2013, 863860, 12.06.2013.

Research output: Contribution to journalArticle

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