TY - JOUR
T1 - Comparative transcriptome analysis reveals a potential photosynthate partitioning mechanism between lipid and starch biosynthetic pathways in green microalgae
AU - Chang, Wen Chi
AU - Zheng, Han Qin
AU - Chen, Ching Nen Nathan
N1 - Funding Information:
The authors thank the High-throughput Genome Analysis Core Facility of National Core Facility Program for Biotechnology, Taiwan (NSC-100-2319-B-010-001) for GS FLX sequencing. This work was supported in part by grants NSC 102-2221-E-110-053-MY2 to Ching-Nen Nathan Chen, and MOST 103-2311-B-006 -001 , NSC 102-2313-B-006-004 and NSC 99-2628-B-006-016-MY3 to Wen-Chi Chang from the Ministry of Science and Technology, Taiwan . This work was also financed by the Top University grant from the Ministry of Education, Taiwan .
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Comparative transcriptome analysis provides a tool to top-view regulations of biochemical pathways in the cells and predicts the key enzymes by comparing gene expression changes in different conditions. Transcriptomes of a green microalga Neodesmus sp. UTEX 2219-4 treated under different stresses favorable or unfavorable for lipid biosynthesis were sequenced and compared to elucidate the photosynthate partitioning mechanism between fatty acid and starch biosynthesis pathways. This microalga contains 20,556 isotigs, and 8387 of these have homologs in the Non-Redundant (NR) database. The fatty acid and starch biosynthetic pathways of this microalga were reconstructed. The expression levels of the isotigs related to these pathways and involved in photosynthesis were calculated. The photosynthetic light reaction genes were down-regulated but electron carriers were up-regulated under osmotic and salt stresses, suggesting that ATP production was supported by cyclic electron transport in these conditions. In lipid producing conditions, expression of multiple isotigs in the fatty acid biosynthesis pathway was up-regulated, indicating that overexpression of acetyl-CoA carboxylase alone was not sufficient to enhance lipid biosynthesis. Results from this study suggested that triose phosphate isomerase is the key enzyme regulating photosynthate partitioning between fatty acid and starch biosyntheses in green microalgae.
AB - Comparative transcriptome analysis provides a tool to top-view regulations of biochemical pathways in the cells and predicts the key enzymes by comparing gene expression changes in different conditions. Transcriptomes of a green microalga Neodesmus sp. UTEX 2219-4 treated under different stresses favorable or unfavorable for lipid biosynthesis were sequenced and compared to elucidate the photosynthate partitioning mechanism between fatty acid and starch biosynthesis pathways. This microalga contains 20,556 isotigs, and 8387 of these have homologs in the Non-Redundant (NR) database. The fatty acid and starch biosynthetic pathways of this microalga were reconstructed. The expression levels of the isotigs related to these pathways and involved in photosynthesis were calculated. The photosynthetic light reaction genes were down-regulated but electron carriers were up-regulated under osmotic and salt stresses, suggesting that ATP production was supported by cyclic electron transport in these conditions. In lipid producing conditions, expression of multiple isotigs in the fatty acid biosynthesis pathway was up-regulated, indicating that overexpression of acetyl-CoA carboxylase alone was not sufficient to enhance lipid biosynthesis. Results from this study suggested that triose phosphate isomerase is the key enzyme regulating photosynthate partitioning between fatty acid and starch biosyntheses in green microalgae.
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U2 - 10.1016/j.algal.2016.03.007
DO - 10.1016/j.algal.2016.03.007
M3 - Article
AN - SCOPUS:84960125994
VL - 16
SP - 54
EP - 62
JO - Algal Research
JF - Algal Research
SN - 2211-9264
ER -