Comparative transcriptomics provides insight into floral color polymorphism in a pleione limprichtii orchid population

Yiyi Zhang, Tinghong Zhou, Zhongwu Dai, Xiaoyu Dai, Wei Li, Mengxia Cao, Chengru Li, Wen Chieh Tsai, Xiaoqian Wu, Junwen Zhai, Zhongjian Liu, Shasha Wu

Research output: Contribution to journalArticle

Abstract

Floral color polymorphism can provide great insight into species evolution from a genetic and ecological standpoint. Color variations between species are often mediated by pollinators and are fixed characteristics, indicating their relevance to adaptive evolution, especially between plants within a single population or between similar species. The orchid genus Pleione has a wide variety of flower colors, from violet, rose-purple, pink, to white, but their color formation and its evolutionary mechanism are unclear. Here, we selected the P. limprichtii population in Huanglong, Sichuan Province, China, which displayed three color variations: Rose-purple, pink, and white, providing ideal material for exploring color variations with regard to species evolution. We investigated the distribution pattern of the different color morphs. The ratio of rose-purple:pink:white-flowered individuals was close to 6:3:1. We inferred that the distribution pattern may serve as a reproductive strategy to maintain the population size. Metabolome analysis was used to reveal that cyanindin derivatives and delphidin are the main color pigments involved. RNA sequencing was used to characterize anthocyanin biosynthetic pathway-related genes and reveal different color formation pathways and transcription factors in order to identify differentially-expressed genes and explore their relationship with color formation. In addition, qRT-PCR was used to validate the expression patterns of some of the genes. The results show that PlFLS serves as a crucial gene that contributes to white color formation and that PlANS and PlUFGT are related to the accumulation of anthocyanin which is responsible for color intensity, especially in pigmented flowers. Phylogenetic and co-expression analyses also identified a R2R3-MYB gene PlMYB10, which is predicted to combine with PlbHLH20 or PlbHLH26 along with PlWD40-1 to form an MBW protein complex (MYB, bHLH, and WDR) that regulates PlFLS expression and may serve as a repressor of anthocyanin accumulation-controlled color variations. Our results not only explain the molecular mechanism of color variation in P. limprichtii, but also contribute to the exploration of a flower color evolutionary model in Pleione, as well as other flowering plants.

Original languageEnglish
Article number247
JournalInternational journal of molecular sciences
Volume21
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

Fingerprint

polymorphism
Polymorphism
Color
color
Population
genes
Anthocyanins
Genes
distribution (property)
RNA Sequence Analysis
Transcription factors
sequencing
Metabolome
Molecular Evolution
Biosynthetic Pathways
Population Density
pigments
RNA
Pigments
China

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Zhang, Yiyi ; Zhou, Tinghong ; Dai, Zhongwu ; Dai, Xiaoyu ; Li, Wei ; Cao, Mengxia ; Li, Chengru ; Tsai, Wen Chieh ; Wu, Xiaoqian ; Zhai, Junwen ; Liu, Zhongjian ; Wu, Shasha. / Comparative transcriptomics provides insight into floral color polymorphism in a pleione limprichtii orchid population. In: International journal of molecular sciences. 2020 ; Vol. 21, No. 1.
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Comparative transcriptomics provides insight into floral color polymorphism in a pleione limprichtii orchid population. / Zhang, Yiyi; Zhou, Tinghong; Dai, Zhongwu; Dai, Xiaoyu; Li, Wei; Cao, Mengxia; Li, Chengru; Tsai, Wen Chieh; Wu, Xiaoqian; Zhai, Junwen; Liu, Zhongjian; Wu, Shasha.

In: International journal of molecular sciences, Vol. 21, No. 1, 247, 01.01.2020.

Research output: Contribution to journalArticle

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T1 - Comparative transcriptomics provides insight into floral color polymorphism in a pleione limprichtii orchid population

AU - Zhang, Yiyi

AU - Zhou, Tinghong

AU - Dai, Zhongwu

AU - Dai, Xiaoyu

AU - Li, Wei

AU - Cao, Mengxia

AU - Li, Chengru

AU - Tsai, Wen Chieh

AU - Wu, Xiaoqian

AU - Zhai, Junwen

AU - Liu, Zhongjian

AU - Wu, Shasha

PY - 2020/1/1

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