Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

Li Wei Cheng; Sheng Wen Huang; Li Min Huang; Luan Yin Chang; Pei Lan Shao; David Kiang; Jen Ren Wang

doi:10.1016/j.jviromet.2012.03.004

Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

Li Wei Cheng, Sheng Wen Huang, Li Min Huang, Luan Yin Chang, Pei Lan Shao, David Kiang, Jen Ren Wang

Department of Medical Laboratory Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	Journal of Virological Methods
Volume	182
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2012.03.004
Publication status	Published - 2012 Jun

All Science Journal Classification (ASJC) codes

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.jviromet.2012.03.004

Cite this

@article{47b45f2c0a6b45deb4a4436f75a0251f,

title = "Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine",

abstract = "Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.",

author = "Cheng, {Li Wei} and Huang, {Sheng Wen} and Huang, {Li Min} and Chang, {Luan Yin} and Shao, {Pei Lan} and David Kiang and Wang, {Jen Ren}",

note = "Funding Information: This work was supported by the Taiwan Center for Disease Control grants; National Health Research Institutes grants; the National Science Council grant NSC 100-2321-B-002-013. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2012",

month = jun,

doi = "10.1016/j.jviromet.2012.03.004",

language = "English",

volume = "182",

pages = "43--49",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

AU - Cheng, Li Wei

AU - Huang, Sheng Wen

AU - Huang, Li Min

AU - Chang, Luan Yin

AU - Shao, Pei Lan

AU - Kiang, David

AU - Wang, Jen Ren

N1 - Funding Information: This work was supported by the Taiwan Center for Disease Control grants; National Health Research Institutes grants; the National Science Council grant NSC 100-2321-B-002-013. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/6

Y1 - 2012/6

N2 - Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.

AB - Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.

UR - http://www.scopus.com/inward/record.url?scp=84862783922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862783922&partnerID=8YFLogxK

U2 - 10.1016/j.jviromet.2012.03.004

DO - 10.1016/j.jviromet.2012.03.004

M3 - Article

C2 - 22433514

AN - SCOPUS:84862783922

SN - 0166-0934

VL - 182

SP - 43

EP - 49

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this