Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

Li Wei Cheng; Sheng Wen Huang; Li Min Huang; Luan Yin Chang; Pei Lan Shao; David Kiang; Jen Ren Wang

doi:10.1016/j.jviromet.2012.03.004

Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

Li Wei Cheng
, Sheng Wen Huang
, Li Min Huang
, Luan Yin Chang
, Pei Lan Shao
, David Kiang
, Jen Ren Wang

Department of Medical Laboratory Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	Journal of Virological Methods
Volume	182
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2012.03.004
Publication status	Published - 2012 Jun

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Virology

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10.1016/j.jviromet.2012.03.004

Cite this

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title = "Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine",

abstract = "Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.",

author = "Cheng, \{Li Wei\} and Huang, \{Sheng Wen\} and Huang, \{Li Min\} and Chang, \{Luan Yin\} and Shao, \{Pei Lan\} and David Kiang and Wang, \{Jen Ren\}",

note = "Funding Information: This work was supported by the Taiwan Center for Disease Control grants; National Health Research Institutes grants; the National Science Council grant NSC 100-2321-B-002-013. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2012",

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doi = "10.1016/j.jviromet.2012.03.004",

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AU - Huang, Sheng Wen

AU - Huang, Li Min

AU - Chang, Luan Yin

AU - Shao, Pei Lan

AU - Kiang, David

AU - Wang, Jen Ren

N1 - Funding Information: This work was supported by the Taiwan Center for Disease Control grants; National Health Research Institutes grants; the National Science Council grant NSC 100-2321-B-002-013. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

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N2 - Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.

AB - Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.

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Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

Abstract

UN SDGs

All Science Journal Classification (ASJC) codes

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