Comparison of the MB/BacT and BACTEC MGIT 960 system for recovery of mycobacteria from clinical specimens

Jing Jou Yan, Ah Huey Huang, Shu Huei Tsai, Wen Chien Ko, Ying Tai Jin, Jiunn Jong Wu

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28 Citations (Scopus)


A total of 543 specimens were cultured in parallel with the MB/BacT and BACTEC MGIT 960 systems and on the conventional solid media. Mycobacteria were identified from 95 (17.5%) specimens, including 63 (66.3%) Mycobacterium tuberculosis and 32 (33.7%) nontuberculous mycobacteria. The recovery rates for the MB/BacT, MGIT 960, and solid media were 91.6, 87.4, and 54.7%, respectively, for all mycobacteria; the recovery rates were 93.6, 88.9, and 63.4%, respectively, for M. tuberculosis complex alone, and 87.5, 84.4, and 37.5%, respectively, for all nontuberculous mycobacteria. The mean times to detection of all mycobacteria by individual systems were 13.9, 8.7, 31.7 days for the MB/BacT, MGIT 960 and solid media, respectively, 13.9, 9.3, 32.9 days for M. tuberculosis alone, and 14.1, 8.1, 27.2 days for all nontuberculous mycobacteria. The contamination rates of the MB/BacT and MGIT 960 were 10.2 and 5.4%, respectively. With regard to detection times and recovery rates, both automated systems are superior to the conventional media (all p < 0.005). As compared to the MB/BacT, the MGIT 960 detected mycobacterial growth more rapidly (p < 0.001), and had a lower contamination rate (p = 0.003); however, there was no statistically significant difference in recovery rates between these two systems. These results indicate that both MGIT 960 and MB/BacT systems are rapid, sensitive, and efficient methods for the recovery of mycobacteria from clinical specimens. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Issue number1
Publication statusPublished - 2000 May

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases


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