Comparisons of neurotransmitter concentrations in the synaptosomal preparation of the normotensive and hypertensive rat urinary bladder

Yat-Ching Tong, Ying Cho Hung, Shinn Nan Lin, Juei Tang Cheng

Research output: Contribution to journalArticle

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Abstract

Synaptosome-rich fractions were prepared from tissue homogenate of the urinary bladder of the spontaneously hypertensive rat and normotensive Wistar-Kyoto rat by differential centrifugation (1000 x g, 17,000 x g and 100,000 x g) with discontinuous sucrose gradient. Synaptosomal acetylcholine, norepinephrine, epinephrine and dopamine were measured by the method of high-performance liquid chromatography. The respective neurotransmitter concentrations for the normotensive rats were 300.4 ± 30.1, 962.8 ± 58.5, 617.3 ± 59.8, and 1354.8 ± 144.2 pmol/mg synaptosomal protein. For the hypertensive rats, the acetylcholine concentration (203.8 ± 23.0 pmol/mg protein) was significantly lower (P < 0.05), while the norepinephrine, epinephrine and dopamine concentrations (1459.0 ± 180.3, 971.3 ± 62.2, and 2161.0 ± 243.4 pmol/mg protein, respectively) were significantly higher (P < 0.05 for all) than those of the normotensive rats. In conclusion, it has been demonstrated that the vesicle-bound catecholamines in the synaptosome-rich fraction of the urinary bladder were significantly increased in hypertensive rats. On the contrary, the synaptosomal acetylcholine concentration was significantly decreased. These findings are suggestive of increased sympathetic innervation and decreased parasympathetic innervation in the urinary bladder of the spontaneously hypertensive rat.

Original languageEnglish
Pages (from-to)141-143
Number of pages3
JournalNeuroscience Letters
Volume204
Issue number1-2
DOIs
Publication statusPublished - 1996 Feb 2

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Neurotransmitter Agents
Urinary Bladder
Acetylcholine
Synaptosomes
Inbred SHR Rats
Epinephrine
Dopamine
Norepinephrine
Proteins
Inbred WKY Rats
Centrifugation
Catecholamines
Sucrose
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "Comparisons of neurotransmitter concentrations in the synaptosomal preparation of the normotensive and hypertensive rat urinary bladder",
abstract = "Synaptosome-rich fractions were prepared from tissue homogenate of the urinary bladder of the spontaneously hypertensive rat and normotensive Wistar-Kyoto rat by differential centrifugation (1000 x g, 17,000 x g and 100,000 x g) with discontinuous sucrose gradient. Synaptosomal acetylcholine, norepinephrine, epinephrine and dopamine were measured by the method of high-performance liquid chromatography. The respective neurotransmitter concentrations for the normotensive rats were 300.4 ± 30.1, 962.8 ± 58.5, 617.3 ± 59.8, and 1354.8 ± 144.2 pmol/mg synaptosomal protein. For the hypertensive rats, the acetylcholine concentration (203.8 ± 23.0 pmol/mg protein) was significantly lower (P < 0.05), while the norepinephrine, epinephrine and dopamine concentrations (1459.0 ± 180.3, 971.3 ± 62.2, and 2161.0 ± 243.4 pmol/mg protein, respectively) were significantly higher (P < 0.05 for all) than those of the normotensive rats. In conclusion, it has been demonstrated that the vesicle-bound catecholamines in the synaptosome-rich fraction of the urinary bladder were significantly increased in hypertensive rats. On the contrary, the synaptosomal acetylcholine concentration was significantly decreased. These findings are suggestive of increased sympathetic innervation and decreased parasympathetic innervation in the urinary bladder of the spontaneously hypertensive rat.",
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Comparisons of neurotransmitter concentrations in the synaptosomal preparation of the normotensive and hypertensive rat urinary bladder. / Tong, Yat-Ching; Hung, Ying Cho; Lin, Shinn Nan; Cheng, Juei Tang.

In: Neuroscience Letters, Vol. 204, No. 1-2, 02.02.1996, p. 141-143.

Research output: Contribution to journalArticle

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