Comprehensive Integrated Single‐Cell Whole Transcriptome Analysis Revealed the p‐EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma

Nam Cong Nhat Huynh, Tze Ta Huang, Chi Thi Kim Nguyen, Fang Kuei Lin

Research output: Contribution to journalArticlepeer-review

Abstract

Cancer‐associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p‐ EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single‐cell RNA sequencing datasets to define OSCC CAFs and p‐EMT subpopulations. We highlighted the cell–cell interaction network of CAFs and p‐EMT tumor cells and suggested biomarkers for the diagnosis and prognosis of OSCC during the metastasis condition. The analysis discovered four subtypes of CAFs: one p‐EMT tumor cell population, and cycling tumor cells as well as TNFSF12‐ TNFRSF25/TNFRSF12A interactions between CAFs and p‐EMT tumor cells during tumor metasta-sis. This suggests the prediction of therapeutically targetable checkpoint receptor–ligand interactions between CAFs and p‐EMT tumor cells in OSCC regarding the metastasis status.

Original languageEnglish
Article number6470
JournalInternational journal of molecular sciences
Volume23
Issue number12
DOIs
Publication statusPublished - 2022 Jun 1

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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