TY - JOUR
T1 - Comprehensive Integrated Single‐Cell Whole Transcriptome Analysis Revealed the p‐EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma
AU - Huynh, Nam Cong Nhat
AU - Huang, Tze Ta
AU - Nguyen, Chi Thi Kim
AU - Lin, Fang Kuei
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6/1
Y1 - 2022/6/1
N2 - Cancer‐associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p‐ EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single‐cell RNA sequencing datasets to define OSCC CAFs and p‐EMT subpopulations. We highlighted the cell–cell interaction network of CAFs and p‐EMT tumor cells and suggested biomarkers for the diagnosis and prognosis of OSCC during the metastasis condition. The analysis discovered four subtypes of CAFs: one p‐EMT tumor cell population, and cycling tumor cells as well as TNFSF12‐ TNFRSF25/TNFRSF12A interactions between CAFs and p‐EMT tumor cells during tumor metasta-sis. This suggests the prediction of therapeutically targetable checkpoint receptor–ligand interactions between CAFs and p‐EMT tumor cells in OSCC regarding the metastasis status.
AB - Cancer‐associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p‐ EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single‐cell RNA sequencing datasets to define OSCC CAFs and p‐EMT subpopulations. We highlighted the cell–cell interaction network of CAFs and p‐EMT tumor cells and suggested biomarkers for the diagnosis and prognosis of OSCC during the metastasis condition. The analysis discovered four subtypes of CAFs: one p‐EMT tumor cell population, and cycling tumor cells as well as TNFSF12‐ TNFRSF25/TNFRSF12A interactions between CAFs and p‐EMT tumor cells during tumor metasta-sis. This suggests the prediction of therapeutically targetable checkpoint receptor–ligand interactions between CAFs and p‐EMT tumor cells in OSCC regarding the metastasis status.
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U2 - 10.3390/ijms23126470
DO - 10.3390/ijms23126470
M3 - Article
C2 - 35742914
AN - SCOPUS:85131543417
SN - 1661-6596
VL - 23
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 12
M1 - 6470
ER -