Conductometric characteristic of series pezoelectric quartz crystal system and its application on biochemical assay

Hsien-Chang Chang, B. W. Chang, T. J. Cheng, W. Y. Chang, J. C. Chang

Research output: Contribution to journalArticle

Abstract

In this study, the electrodes inserted into a measuring cell were seriated with an oscillating circuit to construct a series piezoelectric quartz crystal (SPQC) system. Over the measuring range (15 to 6610 μS), the response of oscillating frequency (f) would descend firstly with respect to the increase of solution conductivity (δ) and then change to ascent again for higher conductivity. We found that there was a relatively higher sensitivity (56 Hz/μS)of the SPQC system between 1 and 3 mS. To assess the reliability for the home-made SPQC system, we introduced it for the test of acid-base and precipitation titration and obtained the recovery rates at 98.4 % and 99.0 %, respectively. The SPQC system was also applied for a biochemical assay. As a result, the frequency detection time (FDT) for Escherichia coli (E. coli) could be clearly defined and concluded a linear relationship (from 410 min to 80 min) in one milliliter of medium that contained colony forming unit (CFU) from 10 to 107.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalChinese Journal of Medical and Biological Engineering
Volume17
Issue number1
Publication statusPublished - 1997 Dec 1

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Quartz
Assays
Crystals
Titration
Escherichia coli
Electrodes
Stem Cells
Recovery
Acids
Networks (circuits)

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

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abstract = "In this study, the electrodes inserted into a measuring cell were seriated with an oscillating circuit to construct a series piezoelectric quartz crystal (SPQC) system. Over the measuring range (15 to 6610 μS), the response of oscillating frequency (f) would descend firstly with respect to the increase of solution conductivity (δ) and then change to ascent again for higher conductivity. We found that there was a relatively higher sensitivity (56 Hz/μS)of the SPQC system between 1 and 3 mS. To assess the reliability for the home-made SPQC system, we introduced it for the test of acid-base and precipitation titration and obtained the recovery rates at 98.4 {\%} and 99.0 {\%}, respectively. The SPQC system was also applied for a biochemical assay. As a result, the frequency detection time (FDT) for Escherichia coli (E. coli) could be clearly defined and concluded a linear relationship (from 410 min to 80 min) in one milliliter of medium that contained colony forming unit (CFU) from 10 to 107.",
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Conductometric characteristic of series pezoelectric quartz crystal system and its application on biochemical assay. / Chang, Hsien-Chang; Chang, B. W.; Cheng, T. J.; Chang, W. Y.; Chang, J. C.

In: Chinese Journal of Medical and Biological Engineering, Vol. 17, No. 1, 01.12.1997, p. 65-72.

Research output: Contribution to journalArticle

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