Conformation transitions of adsorbed proteins by interfacial forces at an air-liquid interface and their effect on the catalytic activity of proteins

Ke Hsuan Wang, Wei Dong Lin, Jau Yann Wu, Yuh-Lang Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An efficient method to monitor and control the secondary structures of globular proteins is developed by adsorption of proteins from a bulk solution onto an air-liquid interface. By controlling the concentration of a protein in the aqueous phase, as well as the attractive force exerted by the template layer, the adsorption of proteins can be classified into two stages according to the variation of surface pressure. In the first stage, the proteins adsorb as a single-molecular layer. The interface-molecule interactions induce a structural transition of the adsorbed proteins into a β-sheet conformation (α/β < 0.1). The second stage is initiated by further adsorption of proteins onto the interface, forming multilayer proteins, and triggering a conformational transition into α-helix (α/β > 10). The glucose sensing experiments demonstrate that GOx with α-helix conformation has a much higher sensitivity than β-sheet GOx, attributed to its lower charge transfer resistance at the GOx-electrolyte interface. The present study not only provides a new approach to monitor, control, and design protein conformations, but also raises the importance of adsorption states of proteins in performing bio-activities at bio-interfaces.

Original languageEnglish
Pages (from-to)2717-2722
Number of pages6
JournalSoft Matter
Volume9
Issue number9
DOIs
Publication statusPublished - 2013 Mar 5

Fingerprint

liquid air
Conformations
catalytic activity
Catalyst activity
proteins
Liquids
Air
Proteins
Adsorption
adsorption
Bioactivity
glucose
helices
Electrolytes
Charge transfer
templates
charge transfer
electrolytes
Glucose
Molecules

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

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Conformation transitions of adsorbed proteins by interfacial forces at an air-liquid interface and their effect on the catalytic activity of proteins. / Wang, Ke Hsuan; Lin, Wei Dong; Wu, Jau Yann; Lee, Yuh-Lang.

In: Soft Matter, Vol. 9, No. 9, 05.03.2013, p. 2717-2722.

Research output: Contribution to journalArticle

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