Constituents and anti-multidrug resistance activity of taiwanofungus camphoratus on human cervical cancer cells

Hsin Yi Hung, Chin Chuan Hung, Jun Weil Liang, Chin Fu Chen, Hung Yi Chen, Po Chuen Shieh, Ping Chung Kuo, Tian Shung Wu

Research output: Contribution to journalArticle

Abstract

Resistance to anti-cancer drugs is one of the main factors of treatment failure resulting in high morbidity. Among the reasons of resistance, overexpression of efflux pumps leading to multidrug resistance is an important issue that needs to be solved. Taiwanofungus camphoratus has been used as a nutritional supplement to treat various cancers. However, its effects on the resistance to chemotherapeutic agents are still unknown. In this study, we report four new chemical constituents of T. camphoratus isolated from an ether extract: camphoratins K (1) and N (2) and benzocamphorins G (3) and I (4). Furthermore, we evaluated zhankuic acids A–C for their P-glycoprotein (P-gp) inhibitory effects. The results showed that zhankuic acid A was the most potent P-gp inhibitor compound and (at 20 µM) could reverse drug resistance in human cancer cells, restoring an IC50 of 78.5 nM for doxorubicin, of 48.5 nM for paclitaxel, and of 321.5 nM for vincristine, indicating a reversal fold of 48, 38, and 45 times, respectively. This study provides support for the use of T. camphoratus in the further development of cancer therapy.

Original languageEnglish
Article number3730
JournalMolecules
Volume24
Issue number20
DOIs
Publication statusPublished - 2019 Oct 16

Fingerprint

Multiple Drug Resistance
P-Glycoprotein
Uterine Cervical Neoplasms
cancer
Cells
Vincristine
Paclitaxel
drugs
Pharmaceutical Preparations
Ether
Doxorubicin
Neoplasms
Pumps
Acids
efflux
Treatment Failure
Drug Resistance
acids
Inhibitory Concentration 50
supplements

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Hung, Hsin Yi ; Hung, Chin Chuan ; Liang, Jun Weil ; Chen, Chin Fu ; Chen, Hung Yi ; Shieh, Po Chuen ; Kuo, Ping Chung ; Wu, Tian Shung. / Constituents and anti-multidrug resistance activity of taiwanofungus camphoratus on human cervical cancer cells. In: Molecules. 2019 ; Vol. 24, No. 20.
@article{01c2b73b1fb84275a4a2c39c0bbfeeb7,
title = "Constituents and anti-multidrug resistance activity of taiwanofungus camphoratus on human cervical cancer cells",
abstract = "Resistance to anti-cancer drugs is one of the main factors of treatment failure resulting in high morbidity. Among the reasons of resistance, overexpression of efflux pumps leading to multidrug resistance is an important issue that needs to be solved. Taiwanofungus camphoratus has been used as a nutritional supplement to treat various cancers. However, its effects on the resistance to chemotherapeutic agents are still unknown. In this study, we report four new chemical constituents of T. camphoratus isolated from an ether extract: camphoratins K (1) and N (2) and benzocamphorins G (3) and I (4). Furthermore, we evaluated zhankuic acids A–C for their P-glycoprotein (P-gp) inhibitory effects. The results showed that zhankuic acid A was the most potent P-gp inhibitor compound and (at 20 µM) could reverse drug resistance in human cancer cells, restoring an IC50 of 78.5 nM for doxorubicin, of 48.5 nM for paclitaxel, and of 321.5 nM for vincristine, indicating a reversal fold of 48, 38, and 45 times, respectively. This study provides support for the use of T. camphoratus in the further development of cancer therapy.",
author = "Hung, {Hsin Yi} and Hung, {Chin Chuan} and Liang, {Jun Weil} and Chen, {Chin Fu} and Chen, {Hung Yi} and Shieh, {Po Chuen} and Kuo, {Ping Chung} and Wu, {Tian Shung}",
year = "2019",
month = "10",
day = "16",
doi = "10.3390/molecules24203730",
language = "English",
volume = "24",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "20",

}

Constituents and anti-multidrug resistance activity of taiwanofungus camphoratus on human cervical cancer cells. / Hung, Hsin Yi; Hung, Chin Chuan; Liang, Jun Weil; Chen, Chin Fu; Chen, Hung Yi; Shieh, Po Chuen; Kuo, Ping Chung; Wu, Tian Shung.

In: Molecules, Vol. 24, No. 20, 3730, 16.10.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Constituents and anti-multidrug resistance activity of taiwanofungus camphoratus on human cervical cancer cells

AU - Hung, Hsin Yi

AU - Hung, Chin Chuan

AU - Liang, Jun Weil

AU - Chen, Chin Fu

AU - Chen, Hung Yi

AU - Shieh, Po Chuen

AU - Kuo, Ping Chung

AU - Wu, Tian Shung

PY - 2019/10/16

Y1 - 2019/10/16

N2 - Resistance to anti-cancer drugs is one of the main factors of treatment failure resulting in high morbidity. Among the reasons of resistance, overexpression of efflux pumps leading to multidrug resistance is an important issue that needs to be solved. Taiwanofungus camphoratus has been used as a nutritional supplement to treat various cancers. However, its effects on the resistance to chemotherapeutic agents are still unknown. In this study, we report four new chemical constituents of T. camphoratus isolated from an ether extract: camphoratins K (1) and N (2) and benzocamphorins G (3) and I (4). Furthermore, we evaluated zhankuic acids A–C for their P-glycoprotein (P-gp) inhibitory effects. The results showed that zhankuic acid A was the most potent P-gp inhibitor compound and (at 20 µM) could reverse drug resistance in human cancer cells, restoring an IC50 of 78.5 nM for doxorubicin, of 48.5 nM for paclitaxel, and of 321.5 nM for vincristine, indicating a reversal fold of 48, 38, and 45 times, respectively. This study provides support for the use of T. camphoratus in the further development of cancer therapy.

AB - Resistance to anti-cancer drugs is one of the main factors of treatment failure resulting in high morbidity. Among the reasons of resistance, overexpression of efflux pumps leading to multidrug resistance is an important issue that needs to be solved. Taiwanofungus camphoratus has been used as a nutritional supplement to treat various cancers. However, its effects on the resistance to chemotherapeutic agents are still unknown. In this study, we report four new chemical constituents of T. camphoratus isolated from an ether extract: camphoratins K (1) and N (2) and benzocamphorins G (3) and I (4). Furthermore, we evaluated zhankuic acids A–C for their P-glycoprotein (P-gp) inhibitory effects. The results showed that zhankuic acid A was the most potent P-gp inhibitor compound and (at 20 µM) could reverse drug resistance in human cancer cells, restoring an IC50 of 78.5 nM for doxorubicin, of 48.5 nM for paclitaxel, and of 321.5 nM for vincristine, indicating a reversal fold of 48, 38, and 45 times, respectively. This study provides support for the use of T. camphoratus in the further development of cancer therapy.

UR - http://www.scopus.com/inward/record.url?scp=85073617113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073617113&partnerID=8YFLogxK

U2 - 10.3390/molecules24203730

DO - 10.3390/molecules24203730

M3 - Article

C2 - 31623176

AN - SCOPUS:85073617113

VL - 24

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 20

M1 - 3730

ER -