Abstract
In this study, we developed an electrochemical cellular chip for continuous monitoring of histamine release at single cell level. The cells were individually accommodated and stabilized inside a microwell electrode (MWE), and then the released histamine was electrochemically detected by the MWE. We employed KU-812 cells (ECACC no. 90071807) as a cell model, and a spiked amperogram was observed in accordance to the exocytotic event of histamine release. In our experiment, the investigated cells were stimulated with high K+ solution which increases a cytosolic Ca2+ influx. The amperograms (+0.6 V vs. Ag/AgCI) of single KU-812 cell with and without stimulation were compared, and an amperogram with higher spiked current were found in high K+ solution. Our result shows that a feasible application for the cell secretion measurement by using this device.
| Original language | English |
|---|---|
| Title of host publication | 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 |
| Pages | 227-230 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - 2009 |
| Event | 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan Duration: 2009 Oct 18 → 2009 Oct 21 |
Other
| Other | 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 |
|---|---|
| Country | Taiwan |
| City | Tainan |
| Period | 09-10-18 → 09-10-21 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Pharmacology
Cite this
}
Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. / Lai, Kuei Yui; Chang, Ching Yu; Chou, Tsung Che; Chang, Hsien-Chang.
2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009. 2009. p. 227-230 5559080.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip
AU - Lai, Kuei Yui
AU - Chang, Ching Yu
AU - Chou, Tsung Che
AU - Chang, Hsien-Chang
PY - 2009
Y1 - 2009
N2 - In this study, we developed an electrochemical cellular chip for continuous monitoring of histamine release at single cell level. The cells were individually accommodated and stabilized inside a microwell electrode (MWE), and then the released histamine was electrochemically detected by the MWE. We employed KU-812 cells (ECACC no. 90071807) as a cell model, and a spiked amperogram was observed in accordance to the exocytotic event of histamine release. In our experiment, the investigated cells were stimulated with high K+ solution which increases a cytosolic Ca2+ influx. The amperograms (+0.6 V vs. Ag/AgCI) of single KU-812 cell with and without stimulation were compared, and an amperogram with higher spiked current were found in high K+ solution. Our result shows that a feasible application for the cell secretion measurement by using this device.
AB - In this study, we developed an electrochemical cellular chip for continuous monitoring of histamine release at single cell level. The cells were individually accommodated and stabilized inside a microwell electrode (MWE), and then the released histamine was electrochemically detected by the MWE. We employed KU-812 cells (ECACC no. 90071807) as a cell model, and a spiked amperogram was observed in accordance to the exocytotic event of histamine release. In our experiment, the investigated cells were stimulated with high K+ solution which increases a cytosolic Ca2+ influx. The amperograms (+0.6 V vs. Ag/AgCI) of single KU-812 cell with and without stimulation were compared, and an amperogram with higher spiked current were found in high K+ solution. Our result shows that a feasible application for the cell secretion measurement by using this device.
UR - http://www.scopus.com/inward/record.url?scp=77958013478&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77958013478&partnerID=8YFLogxK
U2 - 10.1109/NANOMED.2009.5559080
DO - 10.1109/NANOMED.2009.5559080
M3 - Conference contribution
SN - 9781424455287
SP - 227
EP - 230
BT - 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
ER -