Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip

Kuei Yui Lai, Ching Yu Chang, Tsung Che Chou, Hsien-Chang Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we developed an electrochemical cellular chip for continuous monitoring of histamine release at single cell level. The cells were individually accommodated and stabilized inside a microwell electrode (MWE), and then the released histamine was electrochemically detected by the MWE. We employed KU-812 cells (ECACC no. 90071807) as a cell model, and a spiked amperogram was observed in accordance to the exocytotic event of histamine release. In our experiment, the investigated cells were stimulated with high K+ solution which increases a cytosolic Ca2+ influx. The amperograms (+0.6 V vs. Ag/AgCI) of single KU-812 cell with and without stimulation were compared, and an amperogram with higher spiked current were found in high K+ solution. Our result shows that a feasible application for the cell secretion measurement by using this device.

Original languageEnglish
Title of host publication2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
Pages227-230
Number of pages4
DOIs
Publication statusPublished - 2009
Event2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan
Duration: 2009 Oct 182009 Oct 21

Other

Other2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
CountryTaiwan
CityTainan
Period09-10-1809-10-21

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Histamine Release
Electrodes
Histamine
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

Lai, K. Y., Chang, C. Y., Chou, T. C., & Chang, H-C. (2009). Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. In 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 (pp. 227-230). [5559080] https://doi.org/10.1109/NANOMED.2009.5559080
Lai, Kuei Yui ; Chang, Ching Yu ; Chou, Tsung Che ; Chang, Hsien-Chang. / Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009. 2009. pp. 227-230
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Lai, KY, Chang, CY, Chou, TC & Chang, H-C 2009, Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. in 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009., 5559080, pp. 227-230, 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009, Tainan, Taiwan, 09-10-18. https://doi.org/10.1109/NANOMED.2009.5559080

Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. / Lai, Kuei Yui; Chang, Ching Yu; Chou, Tsung Che; Chang, Hsien-Chang.

2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009. 2009. p. 227-230 5559080.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lai KY, Chang CY, Chou TC, Chang H-C. Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. In 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009. 2009. p. 227-230. 5559080 https://doi.org/10.1109/NANOMED.2009.5559080