Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip

Kuei Yui Lai; Ching Yu Chang; Tsung Che Chou; Hsien-Chang Chang

doi:10.1109/NANOMED.2009.5559080

Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip

Kuei Yui Lai, Ching Yu Chang, Tsung Che Chou, Hsien-Chang Chang

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this study, we developed an electrochemical cellular chip for continuous monitoring of histamine release at single cell level. The cells were individually accommodated and stabilized inside a microwell electrode (MWE), and then the released histamine was electrochemically detected by the MWE. We employed KU-812 cells (ECACC no. 90071807) as a cell model, and a spiked amperogram was observed in accordance to the exocytotic event of histamine release. In our experiment, the investigated cells were stimulated with high K⁺ solution which increases a cytosolic Ca²⁺ influx. The amperograms (+0.6 V vs. Ag/AgCI) of single KU-812 cell with and without stimulation were compared, and an amperogram with higher spiked current were found in high K⁺ solution. Our result shows that a feasible application for the cell secretion measurement by using this device.

Original language	English
Title of host publication	2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
Pages	227-230
Number of pages	4
DOIs	https://doi.org/10.1109/NANOMED.2009.5559080
Publication status	Published - 2009 Dec 1
Event	2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan Duration: 2009 Oct 18 → 2009 Oct 21

Publication series

Name	2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009

Other

Other	2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
Country	Taiwan
City	Tainan
Period	09-10-18 → 09-10-21

All Science Journal Classification (ASJC) codes

Molecular Medicine
Pharmacology

Access to Document

10.1109/NANOMED.2009.5559080

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Lai, K. Y., Chang, C. Y., Chou, T. C., & Chang, H-C. (2009). Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. In 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 (pp. 227-230). [5559080] (2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009). https://doi.org/10.1109/NANOMED.2009.5559080

Lai, Kuei Yui ; Chang, Ching Yu ; Chou, Tsung Che ; Chang, Hsien-Chang. / Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009. 2009. pp. 227-230 (2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009).

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title = "Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip",

abstract = "In this study, we developed an electrochemical cellular chip for continuous monitoring of histamine release at single cell level. The cells were individually accommodated and stabilized inside a microwell electrode (MWE), and then the released histamine was electrochemically detected by the MWE. We employed KU-812 cells (ECACC no. 90071807) as a cell model, and a spiked amperogram was observed in accordance to the exocytotic event of histamine release. In our experiment, the investigated cells were stimulated with high K+ solution which increases a cytosolic Ca2+ influx. The amperograms (+0.6 V vs. Ag/AgCI) of single KU-812 cell with and without stimulation were compared, and an amperogram with higher spiked current were found in high K+ solution. Our result shows that a feasible application for the cell secretion measurement by using this device.",

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Lai, KY, Chang, CY, Chou, TC & Chang, H-C 2009, Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. in 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009., 5559080, 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009, pp. 227-230, 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009, Tainan, Taiwan, 09-10-18. https://doi.org/10.1109/NANOMED.2009.5559080

Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. / Lai, Kuei Yui; Chang, Ching Yu; Chou, Tsung Che; Chang, Hsien-Chang.

2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009. 2009. p. 227-230 5559080 (2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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Lai KY, Chang CY, Chou TC, Chang H-C. Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip. In 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009. 2009. p. 227-230. 5559080. (2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009). https://doi.org/10.1109/NANOMED.2009.5559080