Continuous Photo-hydrogen Production from Acetate Using Rhodopseudomonas Palustris WP 3-5

Kuei Ling Yeh, Chun Yen Chen, Yung-Chong Lou, Jo Shu Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)


Phototropic hydrogen production using components of dark fermentation metabolites (i.e. acetate) is an ideal biohydrogen production route since it enables the highest yield possible (i.e., 12 mol H2/mol hexose) and could also reduce COD content in the dark fermentation effluent to mitigate the environmental burden. In this work, a purple nonsulfur photosynthetic bacterial isolate Rhodopseudomonas palustris WP3-5 was utilized to produce H2. To assess the performance of the photo-H2 production system, the continuous photobioreactor was operated at a hydraulic retention time (HRT) of 48 h under different acetate concentrations. The optimal H2 production efficiency occurred when the acetate concentration was 3000 mg COD/l, leading to an overall H2 production rate and a H2 yield of 20.4 ml/h/l and 0.98 mol H2/mol acetate, respectively. In all tests, the acetate conversion was nearly constant at 87.0±3.1%. Next, the preferable HRT leading to the best H2 production performance was identified. The results show that at an acetate concentration of 3000 mg COD/L, H2 production rate increased with a decrease in HRT, giving the best performance at HRT=12 h with an overall H2 production rate of 42.05 ml/h/l and a H2 yield of 1.39 mol H2/mol acetate. Operation under the aforementioned optimal substrate concentration and HRT, the overall H2 production rate could be enhanced two-fold higher when compared with that obtained from preliminary tests. In addition, the HRT setting was found to play a key role in influencing the performance of continuous H2 production by R. palustris WP3-5. The proposed continuous cultures seem to be a favourable choice of bioreactor strategy, possessing the potential to achieve higher H2 production efficiency with an excellent stability.

Original languageEnglish
Title of host publicationHydrogen Production Technologies - Part 1
EditorsDetlef Stolten, Thomas Grube
PublisherForschungszentrum Julich GmbH
Number of pages5
ISBN (Electronic)9783893366521
Publication statusPublished - 2010
Event18th World Hydrogen Energy Conference, WHEC 2010 - Essen, Germany
Duration: 2010 May 162010 May 21

Publication series

Name18th World Hydrogen Energy Conference 2010, WHEC 2010, Proceedings


Conference18th World Hydrogen Energy Conference, WHEC 2010

All Science Journal Classification (ASJC) codes

  • Energy Engineering and Power Technology
  • Fuel Technology
  • Renewable Energy, Sustainability and the Environment


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