Control of vascular network location in millimeter-sized 3D-tissues by micrometer-sized collagen coated cells

Chun Yen Liu, Michiya Matsusaki, Mitsuru Akashi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Engineering three-dimensional (3D) vascularized constructs remains a central challenge because capillary network structures are important for sufficient oxygen and nutrient exchange to sustain the viability of engineered constructs. However, construction of 3D-tissues at single cell level has yet to be reported. Previously, we established a collagen coating method for fabricating a micrometer-sized collagen matrix on cell surfaces to control cell distance or cell densities inside tissues. In this study, a simple fabrication method is presented for constructing vascular networks in 3D-tissues over micrometer-sized or even millimeter-sized with controlled cell densities. From the results, well vascularized 3D network structures can be observed with a fluorescence label method mixing collagen coated cells and endothelia cells, indicating that constructed ECM rich tissues have the potential for vascularization, which opens up the possibility for various applications in pharmaceutical or tissue engineering fields.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume472
Issue number1
DOIs
Publication statusPublished - 2016 Mar 25

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Control of vascular network location in millimeter-sized 3D-tissues by micrometer-sized collagen coated cells'. Together they form a unique fingerprint.

Cite this