TY - JOUR
T1 - Controlled release of bovine serum albumin from hydroxyapatite microspheres for protein delivery system
AU - Boonsongrit, Yaowalak
AU - Abe, Hiroya
AU - Sato, Kazuyoshi
AU - Naito, Makio
AU - Yoshimura, Masahiro
AU - Ichikawa, Hideki
AU - Fukumori, Yoshinobu
N1 - Funding Information:
This work was partially supported by a Grant-in-Aid for Science Research in the priority area of “Research and Development Project on Advanced Metallic Glasses, Inorganic Materials, and Joining Technology” from the Ministry of Education, Science, Sports and Culture of Japan, the Ministry of Education, Culture, Sports, Science and Technology (“Academic Frontier” Project, 2006-2010) and the Japan Society for the Promotion of Science (Grant-in-Aid for Scientific Research C16590038).
PY - 2008/2/25
Y1 - 2008/2/25
N2 - Desorption behavior of a model protein (bovine serum albumin, BSA) on commercial hydroxyapatite (HAp) microspheres and its control were investigated for protein delivery system. The desorption behavior related strongly to the phosphate concentration in phosphate buffer solution: the amount of desorbed BSA increased when the phosphate concentration increased. In physiological buffer solution, which contains 10 mM phosphate, the initial burst release of BSA was observed: 70% of BSA was rapidly desorbed after 0.5 h, and 80% after 24 h. In contrast, the extremely low release profile of BSA was observed in distilled water. For the controlled release of BSA in physiological condition, the BSA-loaded HAp microspheres were encapsulated with a biodegradable polymer, poly(lactic acid-co-glycolic acid) (PLGA) by a solid-in oil-in water (S/O/W) emulsion solvent evaporation method. The initial burst was significantly reduced, and the BSA release was remarkably prolonged by the encapsulation.
AB - Desorption behavior of a model protein (bovine serum albumin, BSA) on commercial hydroxyapatite (HAp) microspheres and its control were investigated for protein delivery system. The desorption behavior related strongly to the phosphate concentration in phosphate buffer solution: the amount of desorbed BSA increased when the phosphate concentration increased. In physiological buffer solution, which contains 10 mM phosphate, the initial burst release of BSA was observed: 70% of BSA was rapidly desorbed after 0.5 h, and 80% after 24 h. In contrast, the extremely low release profile of BSA was observed in distilled water. For the controlled release of BSA in physiological condition, the BSA-loaded HAp microspheres were encapsulated with a biodegradable polymer, poly(lactic acid-co-glycolic acid) (PLGA) by a solid-in oil-in water (S/O/W) emulsion solvent evaporation method. The initial burst was significantly reduced, and the BSA release was remarkably prolonged by the encapsulation.
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U2 - 10.1016/j.mseb.2007.09.006
DO - 10.1016/j.mseb.2007.09.006
M3 - Article
AN - SCOPUS:38949216321
SN - 0921-5107
VL - 148
SP - 162
EP - 165
JO - Materials Science and Engineering B: Solid-State Materials for Advanced Technology
JF - Materials Science and Engineering B: Solid-State Materials for Advanced Technology
IS - 1-3
ER -