Convenient quantification of methanol concentration detection utilizing an integrated microfluidic chip

Yao Nan Wang, Ruey Jen Yang, Wei Jhong Ju, Ming Chang Wu, Lung Ming Fu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A rapid and simple technique is proposed for methanol concentration detection using a PMMA (Polymethyl-Methacrylate) microfluidic chip patterned using a commercially available CO2 laser scriber. In the proposed device, methanol and methanol oxidase (MOX) are injected into a three-dimensional circular chamber and are mixed via a vortex stirring effect. The mixture is heated to prompt the formation of formaldehyde and is flowed into a rectangular chamber, to which fuchsin-sulphurous acid is then added. Finally, the microchip is transferred to a UV spectrophotometer for methanol detection purposes. The experimental results show that a correlation coefficient of R2 = 0.9940 is obtained when plotting the optical density against the methanol concentration for samples and an accuracy as high as 93.1% are compared with the determined by the high quality gas chromatography with concentrations in the range of 2 ~ 100 ppm. The methanol concentrations of four commercial red wines are successfully detected using the developed device. Overall, the results show that the proposed device provides a rapid and accurate means of detecting the methanol concentration for a variety of applications in the alcoholic beverage inspection and control field.

Original languageEnglish
Article number034111
JournalBiomicrofluidics
Volume6
Issue number3
DOIs
Publication statusPublished - 2012 Jul 18

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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